A Novel Label-free Method to Determine Equilibrium Dissociation Constants of Antibodies Binding to Cell Surface Proteins

Overview

Journal Sci Rep

Date 2025 Jan 27

PMID 39870691

Authors

Eilyn R Lacy

Rupesh Nanjunda

Scott L Klakamp

Deborah Kwok

Jennifer F Nemeth

Gordon D Powers

H Hugo Caicedo

Steven A Jacobs

Affiliations

Soon will be listed here.

Abstract

Solution-based affinity assays are used for the selection and characterization of proteins that could be developed into therapeutic molecules. However, these assays have limitations for cell-surface proteins as in most cases their purification requires detergent solubilization and are unlikely to assume conformations in solution that resemble their native states in cell membranes. This report describes a novel electrochemiluminescence-based method, called MSD-CAT, for the affinity analysis of antibodies binding to cell-surface receptors. MSD-CAT was used to evaluate the binding of monoclonal antibodies, Fab fragments, and bispecific antibodies targeting the cell-surface receptor interleukin 3 receptor alpha (CD123) and the results were compared to data obtained using surface plasmon resonance (SPR). The data showed that MSD-CAT can be successfully applied to determine binding affinity on cells in a label free format and without the need for laborious solubilization procedures to generate recombinant antigen. In addition, this method has the potential for high-throughput application while enabling simultaneous determination of equilibrium dissociation constant (K) and receptor density within the same experiment.

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