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Stability of MiRNA in Human Urine Supports Its Biomarker Potential

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Journal Biomark Med
Date 2013 Aug 3
PMID 23905899
Citations 112
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Abstract

Aim: miRNAs are showing utility as biomarkers in urologic disease, however, a rigorous evaluation of their stability in urine is lacking. Here, we evaluate the stability of miRNAs in urine under clinically relevant storage procedures.

Materials & Methods: Eight healthy individuals provided clean catch urine samples that were stored at room temperature or at 4°C for 5 days, or subjected to ten freeze-thaw cycles at -80°C. For each condition, two miRNAs, miR-16 and miR-21, were quantitated by quantitative real-time PCR.

Results: All conditions demonstrated a surprising degree of stability of miRNAs in the urine: by the end of ten freeze-thaw cycles, 23-37% of the initial amount remained; over the 5-day period of storage at room temperature, 35% of the initial amount remained; and at 4°C, 42-56% of the initial amount remained. Both miRNAs also showed degradation at approximately the same rate.

Conclusion: miRNAs are relatively stable in urine under a variety of storage conditions, which supports their utility as urinary biomarkers.

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