A Single-stranded Junction Modulates Nanosecond Motional Ordering of the Substrate Recognition Duplex of a Group I Ribozyme

Overview

Journal Chembiochem

Specialty Biochemistry

Date 2013 Aug 1

PMID 23900919

Citations 5

Authors

Phuong Nguyen

Xuesong Shi

Snorri Th Sigurdsson

Daniel Herschlag

Peter Z Qin

Affiliations

Soon will be listed here.

Abstract

Rigid spinning: Site-directed spin-labeling studies using a rigid nitroxide spin label (Ç) reveal that both length and sequence of a single-stranded junction (J1/2) modulate nanosecond motional ordering of the substrate-recognition duplex (P1) of the 120 kD group I ribozyme. The studies demonstrate an approach for experimental measurements of nanosecond dynamics in high-molecular-weight RNA complexes.

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