Targeted Enrichment: Maximizing Orthologous Gene Comparisons Across Deep Evolutionary Time

Overview

Journal PLoS One

Specialties General Medicine
Science

Date 2013 Jul 12

PMID 23844125

Citations 18

Authors

Shannon M Hedtke

Matthew J Morgan

David C Cannatella

David M Hillis

Affiliations

Soon will be listed here.

Abstract

Estimated phylogenies of evolutionarily diverse taxa will be well supported and more likely to be historically accurate when the analysis contains large amounts of data-many genes sequenced across many taxa. Inferring such phylogenies for non-model organisms is challenging given limited resources for whole-genome sequencing. We take advantage of genomic data from a single species to test the limits of hybridization-based enrichment of hundreds of exons across frog species that diverged up to 250 million years ago. Enrichment success for a given species depends greatly on the divergence time between it and the reference species, and the resulting alignment contains a significant proportion of missing data. However, our alignment generates a well-supported phylogeny of frogs, suggesting that this technique is a practical solution towards resolving relationships across deep evolutionary time.

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