In Vivo Mapping of Notch Pathway Activity in Normal and Stress Hematopoiesis

Overview

Journal Cell Stem Cell

Publisher Cell Press

Specialty Cell Biology

Date 2013 Jun 25

PMID 23791481

Citations 59

Authors

Philmo Oh

Camille Lobry

Jie Gao

Anastasia Tikhonova

Evangelia Loizou

Jan Manet

Ben Van Handel

Sherif Ibrahim

Jeffrey Greve

Hanna Mikkola

Spyros Artavanis-Tsakonas

Iannis Aifantis

Affiliations

Soon will be listed here.

Abstract

Accumulating evidence suggests that Notch signaling is active at multiple points during hematopoiesis. Until recently, the majority of such studies focused on Notch signaling in lymphocyte differentiation and knowledge of individual Notch receptor roles has been limited due to a paucity of genetic tools available. In this manuscript we generate and describe animal models to identify and fate-map stem and progenitor cells expressing each Notch receptor, delineate Notch pathway activation, and perform in vivo gain- and loss-of-function studies dissecting Notch signaling in early hematopoiesis. These models provide comprehensive genetic maps of lineage-specific Notch receptor expression and activation in hematopoietic stem and progenitor cells. Moreover, they establish a previously unknown role for Notch signaling in the commitment of blood progenitors toward the erythrocytic lineage and link Notch signaling to optimal organismal response to stress erythropoiesis.

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