Rapid Generation of Inducible Mouse Mutants

Overview

Journal Nucleic Acids Res

Publisher Oxford University Press

Specialty Biochemistry

Date 2003 Feb 13

PMID 12582257

Citations 158

Authors

Jost Seibler

Branko Zevnik

Birgit Kuter-Luks

Susanne Andreas

Heidrun Kern

Thomas Hennek

Anja Rode

Cornelia Heimann

Nicole Faust

Gunther Kauselmann

Michael Schoor

Rudolf Jaenisch

Klaus Rajewsky

Ralf Kuhn

Frieder Schwenk

Affiliations

Soon will be listed here.

Abstract

We have generated an optimized inducible recombination system for conditional gene targeting based on a Cre recombinase-steroid receptor fusion. This configuration allows efficient Cre-mediated recombination in most organs of the mouse upon induction, without detectable background activity. An ES cell line, was established that carries the inducible recombinase and a loxP-flanked lacZ reporter gene. Out of this line, completely ES cell-derived mice were efficiently produced through tetraploid blastocyst complementation, without the requirement of mouse breeding. Our findings provide a new concept allowing the generation of inducible mouse mutants within 6 months, as compared to 14 months using the current protocol.

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