Signal Transducer and Activator of Transcription (STAT)-3 Regulates MicroRNA Gene Expression in Chronic Lymphocytic Leukemia Cells

Overview

Journal Mol Cancer

Publisher Biomed Central

Specialties Molecular Biology
Oncology

Date 2013 Jun 4

PMID 23725032

Citations 39

Authors

Uri Rozovski

George A Calin

Tetsuro Setoyama

Lucilla DAbundo

David M Harris

Ping Li

Zhiming Liu

Srdana Grgurevic

Alessandra Ferrajoli

Stefan Faderl

Jan A Burger

Susan OBrien

William G Wierda

Michael J Keating

Zeev Estrov

Affiliations

Soon will be listed here.

Abstract

Backgrounds: Approximately 1,000 microRNAs (miRs) are present in the human genome; however, little is known about the regulation of miR transcription. Because miR levels are deregulated in chronic lymphocytic leukemia (CLL) and signal transducer and activator of transcription (STAT)-3 is constitutively activated in CLL, we sought to determine whether STAT3 affects the transcription of miR genes in CLL cells.

Methods: We used publically available data from the ENCODE project to identify putative STAT3 binding sites in the promoters of miR genes. Then we transfected CLL cells with STAT3-shRNA or with an empty vector, and to determine which miRs are differentially expressed, we used a miR microarray approach followed by validation of the microarray results for 6 miRs using quantitative real-time polymerase chain reaction (qRT-PCR).

Results: We identified putative STAT3 binding sites in 160 promoter regions of 200 miRs, including miR-21, miR-29, and miR-155, whose levels have been reported to be upregulated in CLL. Levels of 72 miRs were downregulated (n = 63) or upregulated (n = 9). qRT-PCR confirmed the array data in 5 of 6 miRs.

Conclusions: The presence of activated STAT3 has a profound effect on miR expression in CLL cells.

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