Frequent Deletions and Down-regulation of Micro- RNA Genes MiR15 and MiR16 at 13q14 in Chronic Lymphocytic Leukemia

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 2002 Nov 16

PMID 12434020

Citations 2103

Authors

George Adrian Calin

Calin Dan Dumitru

Masayoshi Shimizu

Roberta Bichi

Simona Zupo

Evan Noch

Hansjuerg Aldler

Sashi Rattan

Michael Keating

Kanti Rai

Laura Rassenti

Thomas Kipps

Massimo Negrini

Florencia Bullrich

Carlo M Croce

Affiliations

Soon will be listed here.

Abstract

Micro-RNAs (miR genes) are a large family of highly conserved noncoding genes thought to be involved in temporal and tissue-specific gene regulation. MiRs are transcribed as short hairpin precursors ( approximately 70 nt) and are processed into active 21- to 22-nt RNAs by Dicer, a ribonuclease that recognizes target mRNAs via base-pairing interactions. Here we show that miR15 and miR16 are located at chromosome 13q14, a region deleted in more than half of B cell chronic lymphocytic leukemias (B-CLL). Detailed deletion and expression analysis shows that miR15 and miR16 are located within a 30-kb region of loss in CLL, and that both genes are deleted or down-regulated in the majority ( approximately 68%) of CLL cases.

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