Microfluidic Amperometric Sensor for Analysis of Nitric Oxide in Whole Blood

Overview

Journal Anal Chem

Specialty Chemistry

Date 2013 May 23

PMID 23692300

Citations 19

Authors

Rebecca A Hunter

Benjamin J Privett

W Hampton Henley

Elise R Breed

Zhe Liang

Rohit Mittal

Benyam P Yoseph

Jonathan E McDunn

Eileen M Burd

Craig M Coopersmith

J Michael Ramsey

Mark H Schoenfisch

Affiliations

Soon will be listed here.

Abstract

Standard photolithographic techniques and a nitric oxide (NO) selective xerogel polymer were utilized to fabricate an amperometric NO microfluidic sensor with low background noise and the ability to analyze NO levels in small sample volumes (~250 μL). The sensor exhibited excellent analytical performance in phosphate buffered saline, including a NO sensitivity of 1.4 pA nM(-1), a limit of detection (LOD) of 840 pM, and selectivity over nitrite, ascorbic acid, acetaminophen, uric acid, hydrogen sulfide, ammonium, ammonia, and both protonated and deprotonated peroxynitrite (selectivity coefficients of -5.3, -4.2, -4.0, -5.0, -6.0, -5.8, -3.8, -1.5, and -4.0, respectively). To demonstrate the utility of the microfluidic NO sensor for biomedical analysis, the device was used to monitor changes in blood NO levels during the onset of sepsis in a murine pneumonia model.

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