Detection of Specific Antibodies Against Tembusu Virus in Ducks by Use of an E Protein-based Enzyme-linked Immunosorbent Assay

Overview

Journal J Clin Microbiol

Specialty Microbiology

Date 2013 Apr 26

PMID 23616462

Citations 7

Authors

Xiuchen Yin

Rang Lv

Xiaodan Chen

Ming Liu

Ronghong Hua

Yun Zhang

Affiliations

Soon will be listed here.

Abstract

We developed an enzyme-linked immunosorbent assay (ELISA) using eukaryotically expressed E protein as the antigen (termed E-ELISA) to detect antibodies to tembusu virus (TMUV) in ducks. The E-ELISA did not react with antisera to other known pathogens, indicating the E protein is specific for recognizing anti-TMUV antibodies. Compared to the serum neutralization test, the specificity and sensitivity of the E-ELISA was 93.2 and 97.8%, respectively. Therefore, this E-ELISA is a sensitive and rapid method for detecting antibodies against TMUV in ducks.

Citing Articles

Detection of Neutralizing Antibodies to Tembusu Virus: Implications for Infection and Immunity.

Lv J, Yang L, Qu S, Meng R, Li Q, Liu H Front Vet Sci. 2020; 6:442.

PMID: 31921903 PMC: 6914806. DOI: 10.3389/fvets.2019.00442.

The dynamic distribution of duck Tembusu virus in the spleen of infected shelducks.

Sun X, Liu E, Iqbal A, Wang T, Wang X, Haseeb A BMC Vet Res. 2019; 15(1):112.

PMID: 30975151 PMC: 6460551. DOI: 10.1186/s12917-019-1860-6.

Development and application of a monoclonal antibody-based blocking ELISA for detection of antibodies to Tembusu virus in multiple poultry species.

Zhang L, Li Z, Jin H, Hu X, Su J BMC Vet Res. 2018; 14(1):201.

PMID: 29940964 PMC: 6019803. DOI: 10.1186/s12917-018-1537-6.

Structures and Functions of the Envelope Glycoprotein in Flavivirus Infections.

Zhang X, Jia R, Shen H, Wang M, Yin Z, Cheng A Viruses. 2017; 9(11).

PMID: 29137162 PMC: 5707545. DOI: 10.3390/v9110338.

Kinetic analysis of IgM monoclonal antibodies for determination of dengue sample concentration using SPR technique.

Jahanshahi P, Wei Q, Jie Z, Ghomeishi M, Sekaran S, Mahamd Adikan F Bioengineered. 2016; 8(3):239-247.

PMID: 27533620 PMC: 5470514. DOI: 10.1080/21655979.2016.1223413.

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