Diagnosis of Tick-borne Encephalitis by a Mu-capture Immunoglobulin M-enzyme Immunoassay Based on Secreted Recombinant Antigen Produced in Insect Cells

Overview

Journal J Clin Microbiol

Specialty Microbiology

Date 2003 Sep 6

PMID 12958266

Citations 9

Authors

Anu Jaaskelainen

Xiuqi Han

Matthias Niedrig

Antti Vaheri

Olli Vapalahti

Affiliations

Soon will be listed here.

Abstract

Acute tick-borne encephalitis is diagnosed by detection of IgM antibodies to tick-borne encephalitis virus (TBEV) (genus Flavivirus) in patient serum. TBEV membrane (M) and envelope (E) proteins have previously been shown to form virus-like particles when expressed in mammalian cells. We expressed the prM/M and E proteins in insect cells with a recombinant baculovirus system and obtained antigenic protein secreted into the cell culture medium, as evidenced by detection by a panel of five monoclonal antibodies to TBEV E protein. According to the sedimentation pattern in sucrose gradient centrifugation, the proteins were most likely secreted as virus-like particles. A mu-capture immunoglobulin M-enzyme immunoassay (IgM-EIA) test was developed and compared to a commercially available TBEV-IgM test (Progen) based on inactivated purified TBEVs. With a panel of 100 TBEV-IgM-negative, 50 TBEV-IgM-positive, and seven dengue virus-IgM-positive serum samples from our diagnostic laboratory, a sensitivity of 100% and specificity of 99% were obtained, and the correlation coefficient of EIA absorbances with the reference test was 0.93. The antigen was also suitable for IgG antibody detection in an immunofluorescent assay format. This is the first time that secreted, fully antigenic E protein has been produced in insect cells for this arthropod-borne flavivirus.

Citing Articles

Recombinant TBEV Protein E of the Siberian Subtype Is a Candidate Antigen in the ELISA Test System for Differential Diagnosis.

Baryshnikova V, Turchenko Y, Tuchynskaya K, Belyaletdinova I, Butenko A, Dereventsova A Diagnostics (Basel). 2023; 13(20).

PMID: 37892100 PMC: 10606673. DOI: 10.3390/diagnostics13203277.

Plant-Derived Recombinant Vaccines against Zoonotic Viruses.

Zahmanova G, Takova K, Valkova R, Toneva V, Minkov I, Andonov A Life (Basel). 2022; 12(2).

PMID: 35207444 PMC: 8878793. DOI: 10.3390/life12020156.

Multi-laboratory evaluation of ReaScan TBE IgM rapid test, 2016 to 2017.

Albinsson B, Jaaskelainen A, Varv K, Jelovsek M, GeurtsvanKessel C, Vene S Euro Surveill. 2020; 25(12).

PMID: 32234120 PMC: 7118343. DOI: 10.2807/1560-7917.ES.2020.25.12.1900427.

Immunoassay for serodiagnosis of Zika virus infection based on time-resolved Förster resonance energy transfer.

Kareinen L, Hepojoki S, Huhtamo E, Korhonen E, Schmidt-Chanasit J, Hedman K PLoS One. 2019; 14(7):e0219474.

PMID: 31335898 PMC: 6650140. DOI: 10.1371/journal.pone.0219474.

Identification of linear human B-cell epitopes of tick-borne encephalitis virus.

Kuivanen S, Hepojoki J, Vene S, Vaheri A, Vapalahti O Virol J. 2014; 11:115.

PMID: 24946852 PMC: 4078944. DOI: 10.1186/1743-422X-11-115.

References

Han X, Aho M, Vene S, Peltomaa M, Vaheri A, Vapalahti O . Prevalence of tick-borne encephalitis virus in Ixodes ricinus ticks in Finland. J Med Virol. 2001; 64(1):21-8. DOI: 10.1002/jmv.1012. View

BRUMMER-KORVENKONTIO M, Saikku P, Korhonen P, OKER-BLOM N . Arboviruses in Finland. I. Isolation of tick-borne encephalitis (TBE) virus from arthropods, vertebrates, and patients. Am J Trop Med Hyg. 1973; 22(3):382-9. View

Ecker M, Allison S, Meixner T, Heinz F . Sequence analysis and genetic classification of tick-borne encephalitis viruses from Europe and Asia. J Gen Virol. 1999; 80 ( Pt 1):179-185. DOI: 10.1099/0022-1317-80-1-179. View

Konishi E, Fujii A, Mason P . Generation and characterization of a mammalian cell line continuously expressing Japanese encephalitis virus subviral particles. J Virol. 2001; 75(5):2204-12. PMC: 114804. DOI: 10.1128/JVI.75.5.2204-2212.2001. View

Haglund M, Forsgren M, Lindh G, Lindquist L . A 10-year follow-up study of tick-borne encephalitis in the Stockholm area and a review of the literature: need for a vaccination strategy. Scand J Infect Dis. 1996; 28(3):217-24. DOI: 10.3109/00365549609027160. View

Allison S, Stadler K, Mandl C, Kunz C, Heinz F . Synthesis and secretion of recombinant tick-borne encephalitis virus protein E in soluble and particulate form. J Virol. 1995; 69(9):5816-20. PMC: 189449. DOI: 10.1128/JVI.69.9.5816-5820.1995. View

Tomazic J, Poljak M, Popovic P, Maticic M, Beovic B, Avsic-Zupanc T . Tick-borne encephalitis: possibly a fatal disease in its acute stage. PCR amplification of TBE RNA from postmortem brain tissue. Infection. 1997; 25(1):41-3. DOI: 10.1007/BF02113507. View

Lundkvist k , Vene S, Golovljova I, Mavtchoutko V, Forsgren M, Kalnina V . Characterization of tick-borne encephalitis virus from Latvia: evidence for co-circulation of three distinct subtypes. J Med Virol. 2001; 65(4):730-5. DOI: 10.1002/jmv.2097. View

Klockmann U, Krivanec K, Stephenson J, Hilfenhaus J . Protection against European isolates of tick-borne encephalitis virus after vaccination with a new tick-borne encephalitis vaccine. Vaccine. 1991; 9(3):210-2. DOI: 10.1016/0264-410x(91)90157-2. View

10.

HUNT A, Cropp C, Chang G . A recombinant particulate antigen of Japanese encephalitis virus produced in stably-transformed cells is an effective noninfectious antigen and subunit immunogen. J Virol Methods. 2001; 97(1-2):133-49. DOI: 10.1016/s0166-0934(01)00346-9. View

11.

Mandl C, Heinz F, Kunz C . Sequence of the structural proteins of tick-borne encephalitis virus (western subtype) and comparative analysis with other flaviviruses. Virology. 1988; 166(1):197-205. DOI: 10.1016/0042-6822(88)90161-4. View

12.

Zent O, Beran J, Jilg W, Mach T, Banzhoff A . Clinical evaluation of a polygeline-free tick-borne encephalitis vaccine for adolescents and adults. Vaccine. 2003; 21(7-8):738-41. DOI: 10.1016/s0264-410x(02)00592-3. View

13.

Ferlenghi I, Clarke M, Ruttan T, Allison S, Schalich J, Heinz F . Molecular organization of a recombinant subviral particle from tick-borne encephalitis virus. Mol Cell. 2001; 7(3):593-602. DOI: 10.1016/s1097-2765(01)00206-4. View

14.

Vutuc C, Kunze M . Tick-borne encephalitis in Austria: incidence 1990 and 1991. Eur J Epidemiol. 1994; 10(3):343-4. DOI: 10.1007/BF01719360. View

15.

Gunther G, Haglund M, Lindquist L, Forsgren M, Skoldenberg B . Tick-bone encephalitis in Sweden in relation to aseptic meningo-encephalitis of other etiology: a prospective study of clinical course and outcome. J Neurol. 1997; 244(4):230-8. DOI: 10.1007/s004150050077. View

16.

Vene S, Haglund M, Vapalahti O, Lundkvist A . A rapid fluorescent focus inhibition test for detection of neutralizing antibodies to tick-borne encephalitis virus. J Virol Methods. 1998; 73(1):71-5. DOI: 10.1016/s0166-0934(98)00041-x. View

17.

Dumpis U, Crook D, Oksi J . Tick-borne encephalitis. Clin Infect Dis. 2000; 28(4):882-90. DOI: 10.1086/515195. View

18.

Schmaljohn C, VanderZanden L, Bray M, Custer D, Meyer B, Li D . Naked DNA vaccines expressing the prM and E genes of Russian spring summer encephalitis virus and Central European encephalitis virus protect mice from homologous and heterologous challenge. J Virol. 1997; 71(12):9563-9. PMC: 230264. DOI: 10.1128/JVI.71.12.9563-9569.1997. View

19.

Kaiser R, Holzmann H . Laboratory findings in tick-borne encephalitis--correlation with clinical outcome. Infection. 2000; 28(2):78-84. DOI: 10.1007/s150100050051. View

20.

Chambers T, Hahn C, Galler R, Rice C . Flavivirus genome organization, expression, and replication. Annu Rev Microbiol. 1990; 44:649-88. DOI: 10.1146/annurev.mi.44.100190.003245. View