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Detection of Antibodies Specific to an Antigenic Cell Wall Galactomannoprotein for Serodiagnosis of Aspergillus Fumigatus Aspergillosis

Overview
Journal J Clin Microbiol
Specialty Microbiology
Date 2002 May 31
PMID 12037061
Citations 22
Authors
Che-Man Chan
Patrick C Y Woo
Andy S P Leung
Susanna K P Lau
Xiao-Yan Che
Liang Cao
Kwok-Yung Yuen
Affiliations
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Abstract

Aspergilloma and invasive aspergillosis are important opportunistic infections caused by Aspergillus species, among which Aspergillus fumigatus is the most common species associated with human disease. We developed an enzyme-linked immunosorbent assay (ELISA)-based antibody assay with Afmp1p, a purified recombinant antigenic cell wall galactomannoprotein of A. fumigatus. Evaluation of the test with guinea pig sera against A. fumigatus and other pathogenic fungi indicated that this assay was specific for A. fumigatus. Clinical evaluation revealed that the assay was 100% sensitive for patients with aspergilloma and 33.3% sensitive for patients with invasive aspergillosis. No false-positive results were found for serum samples from 80 healthy blood donors, 6 patients with typhoid fever, 4 patients with melioidosis, 20 patients with penicilliosis marneffei, 5 patients with candidiasis, and 4 patients with cryptococcosis, indicating a high specificity of the test. Thus, this ELISA-based test for the detection of anti-Afmp1p antibody can be of significant value as a diagnostic for aspergillosis.

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Schubert M, Xue S, Ebel F, Vaggelas A, Krylov V, Nifantiev N Front Cell Infect Microbiol. 2019; 9:234.

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Lam W, Sze K, Ke Y, Tse M, Zhang H, Woo P Infect Immun. 2019; 87(4).

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Mp1p Is a Virulence Factor in Talaromyces (Penicillium) marneffei.

Woo P, Lau S, Lau C, Tung E, Chong K, Yang F PLoS Negl Trop Dis. 2016; 10(8):e0004907.

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