The Pineal Complex in Roman High Avoidance and Roman Low Avoidance Rats

Overview

Journal J Neural Transm Gen Sect

Specialties Neurology
Physiology

Date 1990 Jan 1

PMID 2357328

Citations 1

Authors

A Seidel

J A Sousa Neto

A Huesgen

L Vollrath

B Manz

C Gentsch

M Lichtsteiner

Affiliations

Soon will be listed here.

Abstract

Previous studies have shown that the pineal gland of Roman high avoidance (RHA/Verh) rats is larger than that of Roman low avoidance rats (RLA/Verh). In the present study measurement of enzyme activities (serotonin-N-acetyl-transferase, hydroxyindole-O-methyltransferase) revealed that pineals of RHA/Verh rats are twice as active in melatonin production than pineals of RLA/Verh rats. Indoleamine content was also higher in RHA/Verh rats, whereas noradrenaline content was the same in both lines. When values were expressed per mg protein, these differences disappeared except for N-acetyl-serotonin and noradrenaline which were higher or lower in RHA/Verh rats, respectively. Both lines had higher serum levels of melatonin during the dark phase than during the light phase. However, RHA/Verh rats had increased serum levels as compared to RLA/Verh rats during both day and night. Morphometric analysis of the deep and superficial part of the pineal complex revealed, that the volumes of both parts are enlarged in RHA/Verh rats. Electron microscopic studies of pineals collected during day- and nighttime showed higher numbers of synaptic ribbons per unit area in pineals of RHA/Verh rats. In pineals collected during June synaptic ribbons displayed a day/night rhythm in RHA/Verh rats only, whereas in glands of both lines collected during November no daily changes were found. These results show that closely related but divergently selected rat lines may differ in pineal ultrastructure and pineal function.

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PMID: 1936201 DOI: 10.1007/BF01923338.

References

Wiklund L . Development of serotonin-containing cells and the sympathetic innervation of the habenular region in the rat brain. Cell Tissue Res. 1974; 155(2):231-43. DOI: 10.1007/BF00221357. View

Gentsch C, Lichtsteiner M, Driscoll P, FEER H . Differential hormonal and physiological responses to stress in Roman high- and low-avoidance rats. Physiol Behav. 1982; 28(2):259-63. DOI: 10.1016/0031-9384(82)90072-5. View

Deguchi T, Axelrod J . Sensitive assay for serotonin N-acetyltransferase activity in rat pineal. Anal Biochem. 1972; 50(1):174-9. DOI: 10.1016/0003-2697(72)90496-4. View

Vollrath L, Seidel A, Huesgen A, Manz B, Pollow K, Leiderer P . One millisecond of light suffices to suppress nighttime pineal melatonin synthesis in rats. Neurosci Lett. 1989; 98(3):297-8. DOI: 10.1016/0304-3940(89)90417-5. View

Eranko O, Eranko L . Loss of histochemically demonstrable catecholamines and acetylcholinesterase from sympathetic nerve fibres of the pineal body of the rat after chemical sympathectomy with 6-hydroxydopamine. Histochem J. 1971; 3(5):357-63. DOI: 10.1007/BF01005017. View

Manz B, Kosfeld H, HARBAUER G, Grill H, Pollow K . Radioimmunoassay of human serum serotonin. J Clin Chem Clin Biochem. 1985; 23(10):657-62. DOI: 10.1515/cclm.1985.23.10.657. View

Bignami G . Selection for high rates and low rates of avoidance conditioning in the rat. Anim Behav. 1965; 13(2):221-7. DOI: 10.1016/0003-3472(65)90038-2. View

Vollrath L, Huesgen A, Seidel A, Manz B, Pollow K . Serotonin and melatonin contents in the pineal glands from different stocks and strains of laboratory rats. Z Versuchstierkd. 1989; 32(2):57-63. View

Champney T, Holtorf A, Steger R, Reiter R . Concurrent determination of enzymatic activities and substrate concentrations in the melatonin synthetic pathway within the same rat pineal gland. J Neurosci Res. 1984; 11(1):59-66. DOI: 10.1002/jnr.490110107. View

10.

Moore R . Indolamine metabolism in the intact and denervated pineal, pineal stalk and habenula. Neuroendocrinology. 1975; 19(4):323-30. DOI: 10.1159/000122453. View

11.

Karasek M, Lewinski A, Vollrath L . Precise annual changes in the numbers of "synaptic" ribbons and spherules in the rat pineal gland. J Biol Rhythms. 1988; 3(1):41-8. DOI: 10.1177/074873048800300103. View

12.

Vollrath L, Howe C . Light and drug induced changes of epiphysial synaptic ribbons. Cell Tissue Res. 1976; 165(3):383-90. DOI: 10.1007/BF00222441. View

13.

Driscoll P, Dedek J, Martin J, Baettig K . Regional 5-HT analysis in Roman high- and low-avoidance rats following MAO inhibition. Eur J Pharmacol. 1980; 68(3):373-6. DOI: 10.1016/0014-2999(80)90536-1. View

14.

Ogren S . Analysis of the avoidance learning deficit induced by the serotonin releasing compound p-chloroamphetamine. Brain Res Bull. 1986; 16(5):645-60. DOI: 10.1016/0361-9230(86)90136-x. View

15.

Boeckmann D . Morphological investigation of the deep pineal of the rat. Cell Tissue Res. 1980; 210(2):283-94. DOI: 10.1007/BF00237616. View

16.

Reynolds E . The use of lead citrate at high pH as an electron-opaque stain in electron microscopy. J Cell Biol. 1963; 17:208-12. PMC: 2106263. DOI: 10.1083/jcb.17.1.208. View

17.

LOWRY O, ROSEBROUGH N, FARR A, RANDALL R . Protein measurement with the Folin phenol reagent. J Biol Chem. 1951; 193(1):265-75. View