Rational Design of a Novel Propeptide for Improving Active Production of Streptomyces Griseus Trypsin in Pichia Pastoris

Overview

Journal Appl Environ Microbiol

Specialties Environmental Health
Microbiology

Date 2013 Apr 9

PMID 23563937

Citations 5

Authors

Zhenmin Ling

Yi Liu

Shaolei Teng

Zhen Kang

Jingjing Zhang

Jian Chen

Guocheng Du

Affiliations

Soon will be listed here.

Abstract

Applying in silico simulations and in vitro experiments, the amino acid proline was proved to have a profound influence on Streptomyces griseus trypsinogen, and the hydrogen bond between H(57) and D(102) was found to be crucial for trypsin activity. By introducing an artificial propeptide, IVEF, the titer of trypsin was increased 6.71-fold.

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