Recent Advances of Molecular Toolbox Construction Expand Pichia Pastoris in Synthetic Biology Applications

Overview

Journal World J Microbiol Biotechnol

Publisher Springer

Specialties Biotechnology
Microbiology

Date 2016 Dec 2

PMID 27905091

Citations 16

Authors

Zhen Kang

Hao Huang

Yunfeng Zhang

Guocheng Du

Jian Chen

Affiliations

Soon will be listed here.

Abstract

Pichia pastoris: (reclassified as Komagataella phaffii), a methylotrophic yeast strain has been widely used for heterologous protein production because of its unique advantages, such as readily achievable high-density fermentation, tractable genetic modifications and typical eukaryotic post-translational modifications. More recently, P. pastoris as a metabolic pathway engineering platform has also gained much attention. In this mini-review, we addressed recent advances of molecular toolboxes, including synthetic promoters, signal peptides, and genome engineering tools that established for P. pastoris. Furthermore, the applications of P. pastoris towards synthetic biology were also discussed and prospected especially in the context of genome-scale metabolic pathway analysis.

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