A Single-molecule Analysis Reveals Morphological Targets for Cellulase Synergy

Overview

Journal Nat Chem Biol

Specialties Biochemistry
Biology
Chemistry

Date 2013 Apr 9

PMID 23563526

Citations 22

Authors

Jerome M Fox

Phillip Jess

Rakesh B Jambusaria

Genny M Moo

Jan Liphardt

Douglas S Clark

Harvey W Blanch

Affiliations

Soon will be listed here.

Abstract

The mechanisms of enzyme activity on solid substrates are not well understood. Unlike enzyme catalysis in aqueous solutions, enzyme activity on surfaces is complicated by adsorption steps and structural heterogeneities that make enzyme-substrate interactions difficult to characterize. Cellulase enzymes, which catalyze the depolymerization of cellulose, show binding specificities for different cellulose surface morphologies, but the influence of these specificities on the activity of multienzyme mixtures has remained unclear. We developed a metric to quantify binding-target arrangements determined by photoactivated localization microscopy, and we used that metric to show that combinations of cellulases designed to bind within similar but nonidentical morphologies can have synergistic activity. This phenomenon cannot be explained with the binary crystalline or amorphous classifications commonly used to characterize cellulase-binding targets. Our results reveal a strategy for improving the activity of cellulolytic mixtures and demonstrate a versatile method for investigating protein organization on heterogeneous surfaces.

Citing Articles

Carbohydrate-Binding Modules of Potential Resources: Occurrence in Nature, Function, and Application in Fiber Recognition and Treatment.

Liu Y, Wang P, Tian J, Seidi F, Guo J, Zhu W Polymers (Basel). 2022; 14(9).

PMID: 35566977 PMC: 9100146. DOI: 10.3390/polym14091806.

Fluorescent Imaging of Extracellular Fungal Enzymes Bound onto Plant Cell Walls.

Gacias-Amengual N, Wohlschlager L, Csarman F, Ludwig R Int J Mol Sci. 2022; 23(9).

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Synergic action of an inserted carbohydrate-binding module in a glycoside hydrolase family 5 endoglucanase.

Ye T, Huang K, Ko T, Wu S Acta Crystallogr D Struct Biol. 2022; 78(Pt 5):633-646.

PMID: 35503211 PMC: 9063844. DOI: 10.1107/S2059798322002601.

Fungal cellulases: protein engineering and post-translational modifications.

Zhang R, Cao C, Bi J, Li Y Appl Microbiol Biotechnol. 2021; 106(1):1-24.

PMID: 34889986 DOI: 10.1007/s00253-021-11723-y.

Processive Enzymes Kept on a Leash: How Cellulase Activity in Multienzyme Complexes Directs Nanoscale Deconstruction of Cellulose.

Zajki-Zechmeister K, Kaira G, Eibinger M, Seelich K, Nidetzky B ACS Catal. 2021; 11(21):13530-13542.

PMID: 34777910 PMC: 8576811. DOI: 10.1021/acscatal.1c03465.

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