Imaging the Action of Antimicrobial Peptides on Living Bacterial Cells

Overview

Journal Sci Rep

Specialty Science

Date 2013 Mar 28

PMID 23532056

Citations 29

Authors

Michelle L Gee

Matthew Burton

Alistair Grevis-James

Mohammed Akhter Hossain

Sally McArthur

Enzo A Palombo

John D Wade

Andrew H A Clayton

Affiliations

Soon will be listed here.

Abstract

Antimicrobial peptides hold promise as broad-spectrum alternatives to conventional antibiotics. The mechanism of action of this class of peptide is a topical area of research focused predominantly on their interaction with artificial membranes. Here we compare the interaction mechanism of a model antimicrobial peptide with single artificial membranes and live bacterial cells. The interaction kinetics was imaged using time-lapse fluorescence lifetime imaging of a fluorescently-tagged melittin derivative. Interaction with the synthetic membranes resulted in membrane pore formation. In contrast, the interaction with bacteria led to transient membrane disruption and corresponding leakage of the cytoplasm, but surprisingly with a much reduced level of pore formation. The discovery that pore formation is a less significant part of lipid-peptide interaction in live bacteria highlights the mechanistic complexity of these interactions in living cells compared to simple artificial systems.

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