Down-regulation of Hepatic Stearoyl-CoA Desaturase-1 Expression by Fucoxanthin Via Leptin Signaling in Diabetic/obese KK-A(y) Mice

Overview

Journal Lipids

Specialty Biochemistry

Date 2013 Mar 22

PMID 23516000

Citations 17

Authors

Fumiaki Beppu

Masashi Hosokawa

Mi-Jin Yim

Taro Shinoda

Kazuo Miyashita

Affiliations

Soon will be listed here.

Abstract

Stearoyl-coenzyme A desaturase-1 (SCD1) is a rate-limiting enzyme that catalyzes the biosynthesis of monounsaturated fatty acids from saturated fatty acids. Recently, SCD1 down-regulation has been implicated in the prevention of obesity, and the improvement of insulin and leptin sensitivity. In this study, we examined the effect of fucoxanthin, a marine carotenoid, on hepatic SCD1 in obese mouse models of hyperleptinemia KK-A(y) and leptin-deficiency ob/ob. In KK-A(y) mice, providing a diet containing 0.2 % fucoxanthin for 2 weeks markedly suppressed SCD1 mRNA and protein expressions in the liver. The fatty acid composition of liver lipids was also affected by an observed decrease in the ratio of oleic acid to stearic acid. Furthermore, serum leptin levels were significantly decreased in hyperleptinemia KK-A(y) mice after 2 weeks of fucoxanthin feeding. However, the suppressive effects of fucoxanthin on hepatic SCD1 and body weight gain were not observed in ob/ob mice. These results show that fucoxanthin down-regulates SCD1 expression and alters fatty acid composition of the liver via regulation of leptin signaling in hyperleptinemia KK-A(y) mice but not in leptin-deficient ob/ob mice.

Citing Articles

Research Progress of Natural Products with the Activity of Anti-nonalcoholic Steatohepatitis.

Wang R, Mao Y, Yu C, Rong Z, Wang R, Wang Y Mini Rev Med Chem. 2024; 24(21):1894-1929.

PMID: 38752645 DOI: 10.2174/0113895575306598240503054317.

Effects of Supplementation with Microalgae Extract from (Mi136) to Support Benefits from a Weight Management Intervention in Overweight Women.

Dickerson B, Maury J, Jenkins V, Nottingham K, Xing D, Gonzalez D Nutrients. 2024; 16(7).

PMID: 38613023 PMC: 11013338. DOI: 10.3390/nu16070990.

Brown Algae as Functional Food Source of Fucoxanthin: A Review.

Din N, Mohd Alayudin A, Sofian-Seng N, Rahman H, Mohd Razali N, Lim S Foods. 2022; 11(15).

PMID: 35954003 PMC: 9368577. DOI: 10.3390/foods11152235.

Fucoxanthin and Colorectal Cancer Prevention.

Terasaki M, Kubota A, Kojima H, Maeda H, Miyashita K, Kawagoe C Cancers (Basel). 2021; 13(10).

PMID: 34069132 PMC: 8156579. DOI: 10.3390/cancers13102379.

Fucoxanthin@Polyvinylpyrrolidone Nanoparticles Promoted Oxidative Stress-Induced Cell Death in Caco-2 Human Colon Cancer Cells.

Sui Y, Gu Y, Lu Y, Yu C, Zheng J, Qi H Mar Drugs. 2021; 19(2).

PMID: 33562511 PMC: 7915087. DOI: 10.3390/md19020092.

References

Masuzaki H, Ogawa Y, Aizawa-Abe M, Hosoda K, Suga J, Ebihara K . Glucose metabolism and insulin sensitivity in transgenic mice overexpressing leptin with lethal yellow agouti mutation: usefulness of leptin for the treatment of obesity-associated diabetes. Diabetes. 1999; 48(8):1615-22. DOI: 10.2337/diabetes.48.8.1615. View

Cohen P, Miyazaki M, Socci N, Hagge-Greenberg A, Liedtke W, Soukas A . Role for stearoyl-CoA desaturase-1 in leptin-mediated weight loss. Science. 2002; 297(5579):240-3. DOI: 10.1126/science.1071527. View

Jiang G, Li Z, Liu F, Ellsworth K, Dallas-Yang Q, Wu M . Prevention of obesity in mice by antisense oligonucleotide inhibitors of stearoyl-CoA desaturase-1. J Clin Invest. 2005; 115(4):1030-8. PMC: 1062893. DOI: 10.1172/JCI23962. View

Miyazaki M, Kim Y, Attie A, Ntambi J . The biosynthesis of hepatic cholesterol esters and triglycerides is impaired in mice with a disruption of the gene for stearoyl-CoA desaturase 1. J Biol Chem. 2000; 275(39):30132-8. DOI: 10.1074/jbc.M005488200. View

Miyazaki M, Sampath H, Liu X, Flowers M, Chu K, Dobrzyn A . Stearoyl-CoA desaturase-1 deficiency attenuates obesity and insulin resistance in leptin-resistant obese mice. Biochem Biophys Res Commun. 2009; 380(4):818-22. PMC: 2665189. DOI: 10.1016/j.bbrc.2009.01.183. View

Myers M, Cowley M, Munzberg H . Mechanisms of leptin action and leptin resistance. Annu Rev Physiol. 2007; 70:537-56. DOI: 10.1146/annurev.physiol.70.113006.100707. View

Ntambi J, Miyazaki M . Recent insights into stearoyl-CoA desaturase-1. Curr Opin Lipidol. 2003; 14(3):255-61. DOI: 10.1097/00041433-200306000-00005. View

Woo M, Jeon S, Kim H, Lee M, Shin S, Shin Y . Fucoxanthin supplementation improves plasma and hepatic lipid metabolism and blood glucose concentration in high-fat fed C57BL/6N mice. Chem Biol Interact. 2010; 186(3):316-22. DOI: 10.1016/j.cbi.2010.05.006. View

Miyazaki M, Bruggink S, Ntambi J . Identification of mouse palmitoyl-coenzyme A Delta9-desaturase. J Lipid Res. 2006; 47(4):700-4. DOI: 10.1194/jlr.C500025-JLR200. View

10.

Biddinger S, Miyazaki M, Boucher J, Ntambi J, Kahn C . Leptin suppresses stearoyl-CoA desaturase 1 by mechanisms independent of insulin and sterol regulatory element-binding protein-1c. Diabetes. 2006; 55(7):2032-41. DOI: 10.2337/db05-0742. View

11.

Hosokawa M, Miyashita T, Nishikawa S, Emi S, Tsukui T, Beppu F . Fucoxanthin regulates adipocytokine mRNA expression in white adipose tissue of diabetic/obese KK-Ay mice. Arch Biochem Biophys. 2010; 504(1):17-25. DOI: 10.1016/j.abb.2010.05.031. View

12.

Mauvoisin D, Prevost M, Ducheix S, Arnaud M, Mounier C . Key role of the ERK1/2 MAPK pathway in the transcriptional regulation of the Stearoyl-CoA Desaturase (SCD1) gene expression in response to leptin. Mol Cell Endocrinol. 2010; 319(1-2):116-28. DOI: 10.1016/j.mce.2010.01.027. View

13.

Kaestner K, Ntambi J, Kelly Jr T, Lane M . Differentiation-induced gene expression in 3T3-L1 preadipocytes. A second differentially expressed gene encoding stearoyl-CoA desaturase. J Biol Chem. 1989; 264(25):14755-61. View

14.

Gautron L, Elmquist J . Sixteen years and counting: an update on leptin in energy balance. J Clin Invest. 2011; 121(6):2087-93. PMC: 3104762. DOI: 10.1172/JCI45888. View

15.

Flowers J, Rabaglia M, Schueler K, Flowers M, Lan H, Keller M . Loss of stearoyl-CoA desaturase-1 improves insulin sensitivity in lean mice but worsens diabetes in leptin-deficient obese mice. Diabetes. 2007; 56(5):1228-39. DOI: 10.2337/db06-1142. View

16.

Ntambi J, Miyazaki M, Stoehr J, Lan H, Kendziorski C, Yandell B . Loss of stearoyl-CoA desaturase-1 function protects mice against adiposity. Proc Natl Acad Sci U S A. 2002; 99(17):11482-6. PMC: 123282. DOI: 10.1073/pnas.132384699. View

17.

Rahman S, Dobrzyn A, Lee S, Dobrzyn P, Miyazaki M, Ntambi J . Stearoyl-CoA desaturase 1 deficiency increases insulin signaling and glycogen accumulation in brown adipose tissue. Am J Physiol Endocrinol Metab. 2004; 288(2):E381-7. DOI: 10.1152/ajpendo.00314.2004. View

18.

Tsukui T, Konno K, Hosokawa M, Maeda H, Sashima T, Miyashita K . Fucoxanthin and fucoxanthinol enhance the amount of docosahexaenoic acid in the liver of KKAy obese/diabetic mice. J Agric Food Chem. 2007; 55(13):5025-9. DOI: 10.1021/jf070110q. View

19.

Maeda H, Hosokawa M, Sashima T, Funayama K, Miyashita K . Fucoxanthin from edible seaweed, Undaria pinnatifida, shows antiobesity effect through UCP1 expression in white adipose tissues. Biochem Biophys Res Commun. 2005; 332(2):392-7. DOI: 10.1016/j.bbrc.2005.05.002. View

20.

Bjermo H, Riserus U . Role of hepatic desaturases in obesity-related metabolic disorders. Curr Opin Clin Nutr Metab Care. 2010; 13(6):703-8. DOI: 10.1097/MCO.0b013e32833ec41b. View