Loss of Stearoyl-CoA Desaturase-1 Function Protects Mice Against Adiposity

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 2002 Aug 15

PMID 12177411

Citations 432

Authors

James M Ntambi

Makoto Miyazaki

Jonathan P Stoehr

Hong Lan

Christina M Kendziorski

Brian S Yandell

Yang Song

Paul Cohen

Jeffrey M Friedman

Alan D Attie

Affiliations

Soon will be listed here.

Abstract

Stearoyl-CoA desaturase (SCD) is a central lipogenic enzyme catalyzing the synthesis of monounsaturated fatty acids, mainly oleate (C18:1) and palmitoleate (C16:1), which are components of membrane phospholipids, triglycerides, wax esters, and cholesterol esters. Several SCD isoforms (SCD1-3) exist in the mouse. Here we show that mice with a targeted disruption of the SCD1 isoform have reduced body adiposity, increased insulin sensitivity, and are resistant to diet-induced weight gain. The protection from obesity involves increased energy expenditure and increased oxygen consumption. Compared with the wild-type mice the SCD1-/- mice have increased levels of plasma ketone bodies but reduced levels of plasma insulin and leptin. In the SCD1-/- mice, the expression of several genes of lipid oxidation are up-regulated, whereas lipid synthesis genes are down-regulated. These observations suggest that a consequence of SCD1 deficiency is an activation of lipid oxidation in addition to reduced triglyceride synthesis and storage.

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