Effects of Partial or Complete Laser-assisted Hatching on the Hatching of Mouse Blastocysts and Their Cell Numbers

Overview

Journal Reprod Biol Endocrinol

Publisher Biomed Central

Specialties Endocrinology
Reproductive Medicine

Date 2013 Mar 21

PMID 23510434

Citations 7

Authors

Chanchai Chailert

Usanee Sanmee

Waraporn Piromlertamorn

Sudarat Samchimchom

Teraporn Vutyavanich

Affiliations

Soon will be listed here.

Abstract

Background: It is still debatable whether a full-thickness assisted hatching (AH) is better than the partial zona thinning. In this research, we used a mouse model to study the effect of partial and complete laser-AH on the rate of completely hatched blastocyst and their cell numbers.

Methods: In experiment 1, mouse morulae had 0, 1, 2 or 3 full-thickness openings of 10 microns created in the zona pellucida with an infrared laser beam. In the second experiment, 0, 1 and 2 openings of 20 microns were studied. In the third experiment, a full-thickness opening of 20 microns or quarter-thinning of the zonal circumference to a depth of 90% was compared with non-AH controls.

Results: No difference in blastocyst formation was found in laser-treated groups and in the controls. In experiment 1, the rate of completely hatched blastocysts was significantly lower than the controls. In experiment 2 when the size of the opening was increased, blastocysts completely hatched at a significantly higher rate than that in the controls. In experiment 3, the rate of completely hatched blastocysts was the highest in the full-thickness group. Cell numbers in completely hatched blastocysts from both AH groups were significantly fewer than those in the controls.

Conclusions: Full-thickness opening resulted in a higher rate of completely hatched blastocysts than quarter zonal-thinning and controls, but the cell numbers were significantly decreased.

Citing Articles

Two laser-assisted hatching methods of embryos in ART: a systematic review and meta-analysis.

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Thinning or Opening: A Randomized Sibling-Embryo Pilot Trial on the Efficacy of Two Laser-Assisted Hatching Modes During the Extended Culture of Highly Fragmented Cleavage Embryos.

Zhang L, Zhou Y, Wu Y, Wu L, Li S, Zhang L Front Endocrinol (Lausanne). 2022; 13:927834.

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The Current Practice of Assisted Hatching for Embryos in Fertility Centres: a General Survey.

Liu Y, Jones C, Coward K Reprod Sci. 2022; 29(9):2664-2673.

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From Zygote to Blastocyst: Application of Ultrashort Lasers in the Field of Assisted Reproduction and Developmental Biology.

Ilina I, Sitnikov D Diagnostics (Basel). 2021; 11(10).

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Controlled hatching at the prescribed site using femtosecond laser for zona pellucida drilling at the early blastocyst stage.

Ilina I, Khramova Y, Ivanova A, Filatov M, Silaeva Y, Deykin A J Assist Reprod Genet. 2020; 38(2):517-529.

PMID: 33205358 PMC: 7884559. DOI: 10.1007/s10815-020-01998-x.

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