Effect of Laser-assisted Hatching and Necrotic Blastomere Removal on the Development of Vitrified-warmed Four-cell Mouse Embryos

Overview

Journal J Assist Reprod Genet

Publisher Springer

Specialties Genetics
Reproductive Medicine

Date 2008 Aug 9

PMID 18688708

Citations 3

Authors

Rouhollah Fathi

Mojtaba Rezazadeh Valojerdi

Poopak Eftekhari-Yazdi

Affiliations

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Abstract

Purpose: To investigate the effect of laser-assisted hatching and necrotic blastomere removal on the development of vitrified-warmed mouse embryos.

Methods: The vitrified-warmed four-cell stage mouse embryos were divided into five groups; vitrified intact with no laser-assisted hatching, vitrified intact with laser-assisted hatching, vitrified damaged with neither laser assisted hatching nor necrotic blastomere removal, vitrified damaged with laser-assisted hatching, and vitrified damaged with necrotic blastomere removal. Thereafter blastocyst formation, blastomere and apoptotic cell number within all groups were statistically compared.

Results: The rate of blastocyst formation showed a significant improvement in the group vitrified intact with laser-assisted hatching. However, neither laser-assisted hatching nor necrotic blastomere removal can improve a delayed vitrified-warmed damaged embryos in term of blastocyst formation and total cell number. Nevertheless, apoptotic cell number was significantly reduced after application of both techniques.

Conclusions: Laser-assisted hatching can improve the development of vitrified-warmed intact four-cell stage mouse embryos, whereas necrotic blastomere removal has no significant effect on the development of vitrified-warmed four-cell stage damaged embryos.

Citing Articles

The impacts of the number of prefreeze and postthaw blastomeres on embryo implantation potential: A systematic analysis.

Wang Y, Liu W, Fan L, Li Z, Huang Y, Chen C Medicine (Baltimore). 2020; 99(13):e19591.

PMID: 32221078 PMC: 7220464. DOI: 10.1097/MD.0000000000019591.

WITHDRAWN: Effect of He-Ne laser irradiation on embryonic development in chicken eggs.

El-Hussein A, Kasem M, Elfaham M, Saad A, Hamblin M Prog Biophys Mol Biol. 2019; .

PMID: 31077705 DOI: 10.1016/j.pbiomolbio.2019.05.002.

Effects of partial or complete laser-assisted hatching on the hatching of mouse blastocysts and their cell numbers.

Chailert C, Sanmee U, Piromlertamorn W, Samchimchom S, Vutyavanich T Reprod Biol Endocrinol. 2013; 11:21.

PMID: 23510434 PMC: 3606362. DOI: 10.1186/1477-7827-11-21.

References

Nagy Z, Taylor T, Elliott T, Massey J, Kort H, Shapiro D . Removal of lysed blastomeres from frozen-thawed embryos improves implantation and pregnancy rates in frozen embryo transfer cycles. Fertil Steril. 2005; 84(6):1606-12. DOI: 10.1016/j.fertnstert.2005.06.027. View

Sifer C, Sellami A, Poncelet C, Kulski P, Martin-Pont B, Bottero J . A prospective randomized study to assess the benefit of partial zona pellucida digestion before frozen-thawed embryo transfers. Hum Reprod. 2006; 21(9):2384-9. DOI: 10.1093/humrep/del149. View

Balaban B, Urman B, Yakin K, Isiklar A . Laser-assisted hatching increases pregnancy and implantation rates in cryopreserved embryos that were allowed to cleave in vitro after thawing: a prospective randomized study. Hum Reprod. 2006; 21(8):2136-40. DOI: 10.1093/humrep/del097. View

Tucker M, Cohen J, Massey J, Mayer M, Wiker S, Wright G . Partial dissection of the zona pellucida of frozen-thawed human embryos may enhance blastocyst hatching, implantation, and pregnancy rates. Am J Obstet Gynecol. 1991; 165(2):341-4; discussion 344-5. DOI: 10.1016/0002-9378(91)90088-9. View

Van den Abbeel E, VAN Steirteghem A . Zona pellucida damage to human embryos after cryopreservation and the consequences for their blastomere survival and in-vitro viability. Hum Reprod. 2000; 15(2):373-8. DOI: 10.1093/humrep/15.2.373. View

Chi H, Koo J, Kim M, Chang S, Chung K . Cryopreservation of human embryos using ethylene glycol in controlled slow freezing. Hum Reprod. 2002; 17(8):2146-51. DOI: 10.1093/humrep/17.8.2146. View

Freemann L, Trounson A, Kirby C . Cryopreservation of human embryos: progress on the clinical use of the technique in human in vitro fertilization. J In Vitro Fert Embryo Transf. 1986; 3(1):53-61. DOI: 10.1007/BF01131381. View

Hartshorne G, Wick K, Elder K, Dyson H . Effect of cell number at freezing upon survival and viability of cleaving embryos generated from stimulated IVF cycles. Hum Reprod. 1990; 5(7):857-61. DOI: 10.1093/oxfordjournals.humrep.a137198. View

Ng E, Naveed F, Lau E, Yeung W, Chan C, Tang O . A randomized double-blind controlled study of the efficacy of laser-assisted hatching on implantation and pregnancy rates of frozen-thawed embryo transfer at the cleavage stage. Hum Reprod. 2005; 20(4):979-85. DOI: 10.1093/humrep/deh724. View

10.

Thouas G, Korfiatis N, French A, Jones G, Trounson A . Simplified technique for differential staining of inner cell mass and trophectoderm cells of mouse and bovine blastocysts. Reprod Biomed Online. 2003; 3(1):25-29. DOI: 10.1016/s1472-6483(10)61960-8. View

11.

Alikani M, Olivennes F, Cohen J . Microsurgical correction of partially degenerate mouse embryos promotes hatching and restores their viability. Hum Reprod. 1993; 8(10):1723-8. DOI: 10.1093/oxfordjournals.humrep.a137923. View

12.

Elliott T, Colturato L, Taylor T, Wright G, Kort H, Nagy Z . Lysed cell removal promotes frozen-thawed embryo development. Fertil Steril. 2007; 87(6):1444-9. DOI: 10.1016/j.fertnstert.2006.11.064. View

13.

Liu W, Zheng Y, Luo M, Huang P, Yue L, Wang L . Effects of removal of necrotic blastomeres from mouse cryopreserved embryos on blastocyst formation and hatching. Theriogenology. 2005; 64(5):1114-20. DOI: 10.1016/j.theriogenology.2005.01.007. View

14.

Tao J, Tamis R . Application of assisted hatching for 2-day-old, frozen-thawed embryo transfer in a poor-prognosis population. J Assist Reprod Genet. 1997; 14(2):128-30. PMC: 3454822. DOI: 10.1007/BF02765783. View

15.

Gabrielsen A, Agerholm I, Toft B, Hald F, Petersen K, Aagaard J . Assisted hatching improves implantation rates on cryopreserved-thawed embryos. A randomized prospective study. Hum Reprod. 2004; 19(10):2258-62. DOI: 10.1093/humrep/deh434. View

16.

Uechi H, Tsutsumi O, Morita Y, Takai Y, Taketani Y . Comparison of the effects of controlled-rate cryopreservation and vitrification on 2-cell mouse embryos and their subsequent development. Hum Reprod. 1999; 14(11):2827-32. DOI: 10.1093/humrep/14.11.2827. View

17.

Rezazadeh Valojerdi M, Eftekhari-Yazdi P, Karimian L, Ashtiani S . Effect of laser zona pellucida opening on clinical outcome of assisted reproduction technology in patients with advanced female age, recurrent implantation failure, or frozen-thawed embryos. Fertil Steril. 2007; 90(1):84-91. DOI: 10.1016/j.fertnstert.2007.06.005. View

18.

Shaw J, Ward C, Trounson A . Evaluation of propanediol, ethylene glycol, sucrose and antifreeze proteins on the survival of slow-cooled mouse pronuclear and 4-cell embryos. Hum Reprod. 1995; 10(2):396-402. DOI: 10.1093/oxfordjournals.humrep.a135951. View

19.

Kasai M . Cryopreservation of mammalian embryos. Mol Biotechnol. 1997; 7(2):173-9. DOI: 10.1007/BF02761753. View

20.

Rall W, Reid D, Polge C . Analysis of slow-warming injury of mouse embryos by cryomicroscopical and physiochemical methods. Cryobiology. 1984; 21(1):106-21. DOI: 10.1016/0011-2240(84)90027-0. View