Live Imaging of Multicolor-labeled Cells in Drosophila

Overview

Journal Development

Specialty Biology

Date 2013 Mar 14

PMID 23482495

Citations 33

Authors

Maria Boulina

Hasitha Samarajeewa

James D Baker

Michael D Kim

Akira Chiba

Affiliations

Soon will be listed here.

Abstract

We describe LOLLIbow, a Brainbow-based live imaging system with applications in developmental biology and neurobiology. The development of an animal, including the environmentally sensitive adaptation of its brain, is thought to proceed through continual orchestration among diverse cell types as they divide, migrate, transform and interact with one another within the body. To facilitate direct visualization of such dynamic morphogenesis by individual cells in vivo, we have modified the original Brainbow for Drosophila in which live imaging is practical during much of its development. Our system offers permanent fluorescent labels that reveal fine morphological details of individual cells without requiring dissection or fixation of the samples. It also features a non-invasive means to control the timing of stochastic tricolor cell labeling with a light pulse. We demonstrate applicability of the new system in a variety of settings that could benefit from direct imaging of the developing multicellular organism with single-cell resolution.

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