Imaging Flies by Fluorescence Microscopy: Principles, Technologies, and Applications

Overview

Journal Genetics

Publisher Oxford University Press

Specialty Genetics

Date 2019 Jan 11

PMID 30626639

Citations 23

Authors

Sebastian Dunst

Pavel Tomancak

Affiliations

Soon will be listed here.

Abstract

The development of fluorescent labels and powerful imaging technologies in the last two decades has revolutionized the field of fluorescence microscopy, which is now widely used in diverse scientific fields from biology to biomedical and materials science. Fluorescence microscopy has also become a standard technique in research laboratories working on as a model organism. Here, we review the principles of fluorescence microscopy technologies from wide-field to Super-resolution microscopy and its application in the research field.

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References

Lee T, Luo L . Mosaic analysis with a repressible cell marker for studies of gene function in neuronal morphogenesis. Neuron. 1999; 22(3):451-61. DOI: 10.1016/s0896-6273(00)80701-1. View

Clarkson M, Saint R . A His2AvDGFP fusion gene complements a lethal His2AvD mutant allele and provides an in vivo marker for Drosophila chromosome behavior. DNA Cell Biol. 1999; 18(6):457-62. DOI: 10.1089/104454999315178. View

Henikoff S, Ahmad K, Platero J, van Steensel B . Heterochromatic deposition of centromeric histone H3-like proteins. Proc Natl Acad Sci U S A. 2000; 97(2):716-21. PMC: 15396. DOI: 10.1073/pnas.97.2.716. View

Mollereau B, Wernet M, Beaufils P, Killian D, Pichaud F, Kuhnlein R . A green fluorescent protein enhancer trap screen in Drosophila photoreceptor cells. Mech Dev. 2000; 93(1-2):151-60. DOI: 10.1016/s0925-4773(00)00287-2. View

Gustafsson M . Surpassing the lateral resolution limit by a factor of two using structured illumination microscopy. J Microsc. 2000; 198(Pt 2):82-7. DOI: 10.1046/j.1365-2818.2000.00710.x. View

Rong Y, Golic K . Gene targeting by homologous recombination in Drosophila. Science. 2000; 288(5473):2013-8. DOI: 10.1126/science.288.5473.2013. View

Grieder N, de Cuevas M, Spradling A . The fusome organizes the microtubule network during oocyte differentiation in Drosophila. Development. 2000; 127(19):4253-64. DOI: 10.1242/dev.127.19.4253. View

Greco V, Hannus M, Eaton S . Argosomes: a potential vehicle for the spread of morphogens through epithelia. Cell. 2001; 106(5):633-45. DOI: 10.1016/s0092-8674(01)00484-6. View

Morin X, Daneman R, Zavortink M, Chia W . A protein trap strategy to detect GFP-tagged proteins expressed from their endogenous loci in Drosophila. Proc Natl Acad Sci U S A. 2001; 98(26):15050-5. PMC: 64981. DOI: 10.1073/pnas.261408198. View

10.

Patterson G, Lippincott-Schwartz J . A photoactivatable GFP for selective photolabeling of proteins and cells. Science. 2002; 297(5588):1873-7. DOI: 10.1126/science.1074952. View

11.

Hayashi S, Ito K, Sado Y, Taniguchi M, Akimoto A, Takeuchi H . GETDB, a database compiling expression patterns and molecular locations of a collection of Gal4 enhancer traps. Genesis. 2002; 34(1-2):58-61. DOI: 10.1002/gene.10137. View

12.

Tomancak P, Beaton A, Weiszmann R, Kwan E, Shu S, Lewis S . Systematic determination of patterns of gene expression during Drosophila embryogenesis. Genome Biol. 2003; 3(12):RESEARCH0088. PMC: 151190. DOI: 10.1186/gb-2002-3-12-research0088. View

13.

Cox R, Spradling A . A Balbiani body and the fusome mediate mitochondrial inheritance during Drosophila oogenesis. Development. 2003; 130(8):1579-90. DOI: 10.1242/dev.00365. View

14.

Forrest K, Gavis E . Live imaging of endogenous RNA reveals a diffusion and entrapment mechanism for nanos mRNA localization in Drosophila. Curr Biol. 2003; 13(14):1159-68. DOI: 10.1016/s0960-9822(03)00451-2. View

15.

Prasher D, Eckenrode V, Ward W, Prendergast F, Cormier M . Primary structure of the Aequorea victoria green-fluorescent protein. Gene. 1992; 111(2):229-33. DOI: 10.1016/0378-1119(92)90691-h. View

16.

Shimomura O, Johnson F, Saiga Y . Extraction, purification and properties of aequorin, a bioluminescent protein from the luminous hydromedusan, Aequorea. J Cell Comp Physiol. 1962; 59:223-39. DOI: 10.1002/jcp.1030590302. View

17.

Royou A, Field C, Sisson J, Sullivan W, Karess R . Reassessing the role and dynamics of nonmuscle myosin II during furrow formation in early Drosophila embryos. Mol Biol Cell. 2003; 15(2):838-50. PMC: 329397. DOI: 10.1091/mbc.e03-06-0440. View

18.

Kelso R, Buszczak M, Quinones A, Castiblanco C, Mazzalupo S, Cooley L . Flytrap, a database documenting a GFP protein-trap insertion screen in Drosophila melanogaster. Nucleic Acids Res. 2003; 32(Database issue):D418-20. PMC: 308749. DOI: 10.1093/nar/gkh014. View

19.

Groth A, Fish M, Nusse R, Calos M . Construction of transgenic Drosophila by using the site-specific integrase from phage phiC31. Genetics. 2004; 166(4):1775-82. PMC: 1470814. DOI: 10.1093/genetics/166.4.1775. View

20.

Snapp E, Iida T, Frescas D, Lippincott-Schwartz J, Lilly M . The fusome mediates intercellular endoplasmic reticulum connectivity in Drosophila ovarian cysts. Mol Biol Cell. 2004; 15(10):4512-21. PMC: 519145. DOI: 10.1091/mbc.e04-06-0475. View