Survival and Post-warming in Vitro Competence of Human Oocytes After High Security Closed System Vitrification

Overview

Journal J Assist Reprod Genet

Publisher Springer

Specialties Genetics
Reproductive Medicine

Date 2013 Jan 29

PMID 23354587

Citations 11

Authors

N De Munck

G Verheyen

L Van Landuyt

D Stoop

H van de Velde

Affiliations

Soon will be listed here.

Abstract

Purpose: To compare two vitrification methods and two warming methods for human oocyte vitrification using a high security closed device in terms of survival, fertilization and embryo development.

Methods: For vitrification, oocytes were (1) immediately placed in equilibration solution or (2) they were gradually exposed to the cryoprotectants. For warming, oocytes were placed (1) in a 25 μl preheated (37 °C) thawing solution droplet that was put at room temperature for 1 min once the oocytes were inside or (2) in a 150 μl droplet for 1 minute at 37 °C.

Results: Survival and preimplantation development were significantly lower when warming was performed in a small preheated droplet. There was no significant difference in survival and embryo development between the gradual or direct exposure to cryoprotectants.

Conclusions: Using this high security closed vitrification device a 90 % survival rate can be achieved when the oocytes are immediately warmed in a large volume at 37 °C.

Citing Articles

Duration of cryostorage is not associated with rates of thaw survival, fertilization, blastulation and ploidy, or pregnancy outcomes of vitrified human oocytes.

Roger S, Estevez S, Hernandez-Nieto C, Kripalani S, Ghofranian A, Baird M J Assist Reprod Genet. 2025; 42(2):525-532.

PMID: 39753943 PMC: 11871184. DOI: 10.1007/s10815-024-03350-z.

The new ice age: the promise and challenges of rapid oocyte warming protocols.

Bartolacci A, Albertini D J Assist Reprod Genet. 2024; 41(11):2969-2971.

PMID: 39556268 PMC: 11621260. DOI: 10.1007/s10815-024-03315-2.

Reply: Concerns regarding the study "Comparing the effects of a commercial and a prototype vitrification medium on meiotic spindle morphology and survival rate of mouse oocytes".

Viana I, Vireque A, Navarro P JBRA Assist Reprod. 2023; 27(3):582-583.

PMID: 37579269 PMC: 10712826. DOI: 10.5935/1518-0557.20230016.

The effect of repeated cryopreservation and thawing using cryotip on the clinical outcomes of embryos.

Mizobe Y, Kuwatsuru Y, Kuroki Y, Fukumoto Y, Tokudome M, Moewaki H Reprod Med Biol. 2021; 20(2):176-181.

PMID: 33850450 PMC: 8022090. DOI: 10.1002/rmb2.12365.

High-security closed devices are efficient and safe to protect human oocytes from potential risk of viral contamination during vitrification and storage especially in the COVID-19 pandemic.

Porcu E, Tranquillo M, Notarangelo L, Ciotti P, Calza N, Zuffa S J Assist Reprod Genet. 2021; 38(3):681-688.

PMID: 33432422 PMC: 7799863. DOI: 10.1007/s10815-021-02062-y.

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