Risk of Contamination of Gametes and Embryos During Cryopreservation and Measures to Prevent Cross-Contamination

Overview

Journal Biomed Res Int

Publisher Wiley

Specialties Biomedical Engineering
Biotechnology
General Medicine

Date 2017 Sep 12

PMID 28890894

Citations 15

Authors

Daniel C Joaquim

Eduardo D Borges

Iara G R Viana

Paula A Navarro

Alessandra A Vireque

Affiliations

Soon will be listed here.

Abstract

The introduction and widespread application of vitrification are one of the most important achievements in human assisted reproduction techniques (ART) of the past decade despite controversy and unclarified issues, mostly related to concerns about disease transmission. Guidance documents published by US Food and Drug Administration, which focused on the safety of tissue/organ donations during Zika virus spread in 2016, as well as some reports of virus, bacteria, and fungi survival to cryogenic temperatures, highlighted the need for a review of the way how potentially infectious material is handled and stored in ART-related procedures. It was experimentally demonstrated that cross-contamination between liquid nitrogen (LN) and embryos may occur when infectious agents are present in LN and oocytes/embryos are not protected by a hermetically sealed device. Thus, this review summarizes pertinent data and opinions regarding the potential hazard of infectious transmission through cryopreserved and banked reproductive cells and tissues in LN. Special attention is given to the survival of pathogens in LN, the risk of cross-contamination, vitrification methods, sterility of LN, and the risks associated with the use of straws, cryovials, and storage dewars.

Citing Articles

Technologies for Vitrification Based Cryopreservation.

Amini M, Benson J Bioengineering (Basel). 2023; 10(5).

PMID: 37237578 PMC: 10215456. DOI: 10.3390/bioengineering10050508.

A comprehensive review and update on human fertility cryopreservation methods and tools.

Antonouli S, Di Nisio V, Messini C, Daponte A, Rajender S, Anifandis G Front Vet Sci. 2023; 10:1151254.

PMID: 37143497 PMC: 10151698. DOI: 10.3389/fvets.2023.1151254.

Adverse events, side effects and complications in mesenchymal stromal cell-based therapies.

Baranovskii D, Klabukov I, Arguchinskaya N, Yakimova A, Kisel A, Yatsenko E Stem Cell Investig. 2022; 9:7.

PMID: 36393919 PMC: 9659480. DOI: 10.21037/sci-2022-025.

Resurrecting biodiversity: advanced assisted reproductive technologies and biobanking.

Bolton R, Mooney A, Pettit M, Bolton A, Morgan L, Drake G Reprod Fertil. 2022; 3(3):R121-R146.

PMID: 35928671 PMC: 9346332. DOI: 10.1530/RAF-22-0005.

Epigenetic Alterations in Cryopreserved Human Spermatozoa: Suspected Potential Functional Defects.

Wang W, Todorov P, Pei C, Wang M, Isachenko E, Rahimi G Cells. 2022; 11(13).

PMID: 35805194 PMC: 9266127. DOI: 10.3390/cells11132110.

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