Efficient Measurement of Opsonising Antibodies to Plasmodium Falciparum Merozoites

Overview

Journal PLoS One

Specialties General Medicine
Science

Date 2013 Jan 10

PMID 23300556

Citations 20

Authors

Danika L Hill

Emily M Eriksson

Amandine B Carmagnac

Danny W Wilson

Alan F Cowman

Diana S Hansen

Louis Schofield

Affiliations

Soon will be listed here.

Abstract

Background: Antibodies targeting merozoites are important in protection from malaria. Therefore, merozoite surface proteins are attractive vaccine candidates. There is a need for robust functional assays to investigate mechanisms of acquired immunity and vaccine efficacy. To date, the study of merozoite phagocytosis has been confounded by the complexity and variability of in vitro assays.

Methodology/principal Findings: We have developed a new flow cytometry-based merozoite phagocytosis assay. An optimized merozoite preparation technique produced high yields of merozoites separated from haemozoin. Phagocytosis by the undifferentiated THP-1 monocytic cell line was mediated only by Fc Receptors, and was therefore ideal for studying opsonising antibody responses. The assay showed robust phagocytosis with highly diluted immune sera and strong inter-assay correlation. The assay effectively measured differences in opsonisation-dependent phagocytosis among individuals.

Conclusions/significance: This highly reproducible assay has potential applications in assessing the role of opsonic phagocytosis in naturally acquired immunity and vaccine trials.

Citing Articles

Naturally acquired antibodies from Beninese infants promote Plasmodium falciparum merozoite-phagocytosis by human blood leukocytes: implications for control of asymptomatic malaria infections.

Fall A, Kana I, Dechavanne C, Garcia-Senosiain A, Guitard E, Milet J Malar J. 2022; 21(1):356.

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GMZ2 Vaccine-Induced Antibody Responses, Naturally Acquired Immunity and the Incidence of Malaria in Burkinabe Children.

Dassah S, Adu B, Tiendrebeogo R, Singh S, Arthur F, Sirima S Front Immunol. 2022; 13:899223.

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Improvement of the antibody-dependent respiratory burst assay for assessing protective immune responses to malaria.

Mansourou A, Joos C, Niass O, Diouf B, Tall A, Perraut R Open Biol. 2022; 12(3):210288.

PMID: 35291880 PMC: 8924748. DOI: 10.1098/rsob.210288.

Neutrophils dominate in opsonic phagocytosis of P. falciparum blood-stage merozoites and protect against febrile malaria.

Garcia-Senosiain A, Kana I, Singh S, Das M, Dziegiel M, Hertegonne S Commun Biol. 2021; 4(1):984.

PMID: 34413459 PMC: 8376957. DOI: 10.1038/s42003-021-02511-5.

Treatment of Plasmodium falciparum merozoites with the protease inhibitor E64 and mechanical filtration increases their susceptibility to complement activation.

Stoute J, Landmesser M, Biryukov S PLoS One. 2020; 15(8):e0237786.

PMID: 32822376 PMC: 7442247. DOI: 10.1371/journal.pone.0237786.

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