Glucan-rich Polysaccharides from Pleurotus Sajor-caju (Fr.) Singer Prevents Glucose Intolerance, Insulin Resistance and Inflammation in C57BL/6J Mice Fed a High-fat Diet

Overview

Journal BMC Complement Altern Med

Publisher Biomed Central

Specialty Rehabilitation Medicine

Date 2012 Dec 25

PMID 23259700

Citations 19

Authors

Gowri Kanagasabapathy

Umah Rani Kuppusamy

Sri Nurestri Abd Malek

Mahmood Ameen Abdulla

Kek-Heng Chua

Vikineswary Sabaratnam

Affiliations

Soon will be listed here.

Abstract

Background: Pleurotus sajor-caju (P. sajor-caju) has been extremely useful in the prevention of diabetes mellitus due to its low fat and high soluble fiber content for thousands of years. Insulin resistance is a key component in the development of diabetes mellitus which is caused by inflammation. In this study, we aimed to investigate the in vivo efficacy of glucan-rich polysaccharide of P. sajor-caju (GE) against diabetes mellitus and inflammation in C57BL/6J mice fed a high-fat diet.

Methods: Diabetes was induced in C57BL/6J mice by feeding a high-fat diet. The mice were randomly assigned to 7 groups (n=6 per group). The control groups in this study were ND (for normal diet) and HFD (for high-fat diet). The treated groups were ND240 (for normal diet) (240 mg/kg b.w) and HFD60, HFD120 and HFD240 (for high-fat), where the mice were administrated with three dosages of GE (60, 120, 240 mg GE/kg b.w respectively). Metformin (2 mg/kg b.w) served as positive control. The glucose tolerance test, glucose and insulin levels were measured at the end of 16 weeks. Expressions of genes for inflammatory markers, GLUT-4 and adiponectin in the adipose tissue of the mice were assessed. One-way ANOVA and Duncan's multiple range tests (DMRT) were used to determine the significant differences between groups.

Results: GE treated groups improved the glucose tolerance, attenuated hyperglycemia and hyperinsulinemia in the mice by up-regulating the adiponectin and GLUT-4 gene expressions. The mice in GE treated groups did not develop insulin resistance. GE also down-regulated the expression of inflammatory markers (IL-6, TNF-α, SAA2, CRP and MCP-1) via attenuation of nuclear transcription factors (NF-κB).

Conclusion: Glucan-rich polysaccharide of P. sajor-caju can serve as a potential agent for prevention of glucose intolerance, insulin resistance and inflammation.

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References

Dandona P, Aljada A, Bandyopadhyay A . Inflammation: the link between insulin resistance, obesity and diabetes. Trends Immunol. 2003; 25(1):4-7. DOI: 10.1016/j.it.2003.10.013. View

Terra X, Pallares V, Ardevol A, Blade C, Fernandez-Larrea J, Pujadas G . Modulatory effect of grape-seed procyanidins on local and systemic inflammation in diet-induced obesity rats. J Nutr Biochem. 2010; 22(4):380-7. DOI: 10.1016/j.jnutbio.2010.03.006. View

Simpson R, Shaw J, Zimmet P . The prevention of type 2 diabetes--lifestyle change or pharmacotherapy? A challenge for the 21st century. Diabetes Res Clin Pract. 2003; 59(3):165-80. DOI: 10.1016/s0168-8227(02)00275-9. View

Mohanty P, Aljada A, Ghanim H, Hofmeyer D, Tripathy D, Syed T . Evidence for a potent antiinflammatory effect of rosiglitazone. J Clin Endocrinol Metab. 2004; 89(6):2728-35. DOI: 10.1210/jc.2003-032103. View

Pramanik M, Mondal S, Chakraborty I, Rout D, Islam S . Structural investigation of a polysaccharide (Fr. II) isolated from the aqueous extract of an edible mushroom, Pleurotus sajor-caju. Carbohydr Res. 2005; 340(4):629-36. DOI: 10.1016/j.carres.2004.12.032. View

Ferrante Jr A . Obesity-induced inflammation: a metabolic dialogue in the language of inflammation. J Intern Med. 2007; 262(4):408-14. DOI: 10.1111/j.1365-2796.2007.01852.x. View

Balagopal P, Graham T, Kahn B, Altomare A, Funanage V, George D . Reduction of elevated serum retinol binding protein in obese children by lifestyle intervention: association with subclinical inflammation. J Clin Endocrinol Metab. 2007; 92(5):1971-4. DOI: 10.1210/jc.2006-2712. View

Yumuk V, Hatemi H, Tarakci T, Uyar N, Turan N, Bagriacik N . High prevalence of obesity and diabetes mellitus in Konya, a central Anatolian city in Turkey. Diabetes Res Clin Pract. 2005; 70(2):151-8. DOI: 10.1016/j.diabres.2005.03.030. View

Wang C, Mao X, Wang L, Liu M, Wetzel M, Guan K . Adiponectin sensitizes insulin signaling by reducing p70 S6 kinase-mediated serine phosphorylation of IRS-1. J Biol Chem. 2007; 282(11):7991-6. DOI: 10.1074/jbc.M700098200. View

10.

Shoelson S, Herrero L, Naaz A . Obesity, inflammation, and insulin resistance. Gastroenterology. 2007; 132(6):2169-80. DOI: 10.1053/j.gastro.2007.03.059. View

11.

Slovacek L, Pavlik V, Slovackova B . The effect of sibutramine therapy on occurrence of depression symptoms among obese patients. Nutr Metab Cardiovasc Dis. 2008; 18(8):e43-4. DOI: 10.1016/j.numecd.2008.04.002. View

12.

Umar A, Ahmed Q, Muhammad B, Dogarai B, Soad S . Anti-hyperglycemic activity of the leaves of Tetracera scandens Linn. Merr. (Dilleniaceae) in alloxan induced diabetic rats. J Ethnopharmacol. 2010; 131(1):140-5. DOI: 10.1016/j.jep.2010.06.016. View

13.

Kern P, Di Gregorio G, Lu T, Rassouli N, Ranganathan G . Adiponectin expression from human adipose tissue: relation to obesity, insulin resistance, and tumor necrosis factor-alpha expression. Diabetes. 2003; 52(7):1779-85. DOI: 10.2337/diabetes.52.7.1779. View

14.

Zou C, Shao J . Role of adipocytokines in obesity-associated insulin resistance. J Nutr Biochem. 2007; 19(5):277-86. DOI: 10.1016/j.jnutbio.2007.06.006. View

15.

Roberts C, Sindhu K . Oxidative stress and metabolic syndrome. Life Sci. 2009; 84(21-22):705-12. DOI: 10.1016/j.lfs.2009.02.026. View

16.

Bailey C . Insulin resistance and antidiabetic drugs. Biochem Pharmacol. 1999; 58(10):1511-20. DOI: 10.1016/s0006-2952(99)00191-4. View

17.

Kuhad A, Chopra K . Attenuation of diabetic nephropathy by tocotrienol: involvement of NFkB signaling pathway. Life Sci. 2009; 84(9-10):296-301. DOI: 10.1016/j.lfs.2008.12.014. View

18.

Hamza N, Berke B, Cheze C, Agli A, Robinson P, Gin H . Prevention of type 2 diabetes induced by high fat diet in the C57BL/6J mouse by two medicinal plants used in traditional treatment of diabetes in the east of Algeria. J Ethnopharmacol. 2010; 128(2):513-8. DOI: 10.1016/j.jep.2010.01.004. View

19.

Scheja L, Heese B, Seedorf K . Beneficial effects of IKKε-deficiency on body weight and insulin sensitivity are lost in high fat diet-induced obesity in mice. Biochem Biophys Res Commun. 2011; 407(2):288-94. DOI: 10.1016/j.bbrc.2011.02.137. View

20.

Zhang W, Liu C, Wang P, Sun S, Su W, Zhang H . Puerarin improves insulin resistance and modulates adipokine expression in rats fed a high-fat diet. Eur J Pharmacol. 2010; 649(1-3):398-402. DOI: 10.1016/j.ejphar.2010.09.054. View