Laboratory Detection of Respiratory Viruses by Automated Techniques

Overview

Journal Open Virol J

Publisher Bentham Open

Date 2012 Dec 19

PMID 23248735

Citations 16

Authors

Mercedes Perez-Ruiz

Irene Pedrosa-Corral

Sara Sanbonmatsu-Gamez

Maria Navarro-Mari

Affiliations

Soon will be listed here.

Abstract

Advances in clinical virology for detecting respiratory viruses have been focused on nucleic acids amplification techniques, which have converted in the reference method for the diagnosis of acute respiratory infections of viral aetiology. Improvements of current commercial molecular assays to reduce hands-on-time rely on two strategies, a stepwise automation (semi-automation) and the complete automation of the whole procedure. Contributions to the former strategy have been the use of automated nucleic acids extractors, multiplex PCR, real-time PCR and/or DNA arrays for detection of amplicons. Commercial fully-automated molecular systems are now available for the detection of respiratory viruses. Some of them could convert in point-of-care methods substituting antigen tests for detection of respiratory syncytial virus and influenza A and B viruses. This article describes laboratory methods for detection of respiratory viruses. A cost-effective and rational diagnostic algorithm is proposed, considering technical aspects of the available assays, infrastructure possibilities of each laboratory and clinic-epidemiologic factors of the infection.

Citing Articles

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References

Hall M, DeFrances C, Williams S, Golosinskiy A, Schwartzman A . National Hospital Discharge Survey: 2007 summary. Natl Health Stat Report. 2010; (29):1-20, 24. View

van Leeuwen J, Goossens L, Hendrix R, van der Palen J, Lusthusz A, Thio B . Equal virulence of rhinovirus and respiratory syncytial virus in infants hospitalized for lower respiratory tract infection. Pediatr Infect Dis J. 2011; 31(1):84-6. DOI: 10.1097/INF.0b013e31823345bf. View

Yun B, Kim M, Park J, Choi E, Lee H, Yun C . Viral etiology and epidemiology of acute lower respiratory tract infections in Korean children. Pediatr Infect Dis J. 1995; 14(12):1054-9. DOI: 10.1097/00006454-199512000-00005. View

Navarro-Mari J, Perez-Ruiz M, Galan Montemayor J, Marcos Maeso M, Reina J, de Ona Navarro M . Circulation of other respiratory viruses and viral co-infection during the 2009 pandemic influenza. Enferm Infecc Microbiol Clin. 2012; 30 Suppl 4:25-31. PMC: 7130202. DOI: 10.1016/S0213-005X(12)70101-5. View

Mahony J, Blackhouse G, Babwah J, Smieja M, Buracond S, Chong S . Cost analysis of multiplex PCR testing for diagnosing respiratory virus infections. J Clin Microbiol. 2009; 47(9):2812-7. PMC: 2738055. DOI: 10.1128/JCM.00556-09. View

Perez-Ruiz M, Yeste R, Ruiz-Perez M, Ruiz-Bravo A, de la Rosa-Fraile M, Navarro-Mari J . Testing of diagnostic methods for detection of influenza virus for optimal performance in the context of an influenza surveillance network. J Clin Microbiol. 2007; 45(9):3109-10. PMC: 2045238. DOI: 10.1128/JCM.00697-07. View

van den Hoogen B, Osterhaus D, Fouchier R . Clinical impact and diagnosis of human metapneumovirus infection. Pediatr Infect Dis J. 2004; 23(1 Suppl):S25-32. DOI: 10.1097/01.inf.0000108190.09824.e8. View

Weinberg A, Brewster L, Clark J, Simoes E . Evaluation of R-Mix shell vials for the diagnosis of viral respiratory tract infections. J Clin Virol. 2004; 30(1):100-5. DOI: 10.1016/j.jcv.2003.09.014. View

Mahony J . Detection of respiratory viruses by molecular methods. Clin Microbiol Rev. 2008; 21(4):716-47. PMC: 2570148. DOI: 10.1128/CMR.00037-07. View

10.

Chemaly R, Torres H, Munsell M, Shah D, Rathod D, Bodey G . An adaptive randomized trial of an intermittent dosing schedule of aerosolized ribavirin in patients with cancer and respiratory syncytial virus infection. J Infect Dis. 2012; 206(9):1367-71. PMC: 3466999. DOI: 10.1093/infdis/jis516. View

11.

Chan K, Yam W, Pang C, Chan K, Lam S, Lo K . Comparison of the NucliSens easyMAG and Qiagen BioRobot 9604 nucleic acid extraction systems for detection of RNA and DNA respiratory viruses in nasopharyngeal aspirate samples. J Clin Microbiol. 2008; 46(7):2195-9. PMC: 2446927. DOI: 10.1128/JCM.00315-08. View

12.

Verheyen J, Kaiser R, Bozic M, Timmen-Wego M, Maier B, Kessler H . Extraction of viral nucleic acids: comparison of five automated nucleic acid extraction platforms. J Clin Virol. 2012; 54(3):255-9. DOI: 10.1016/j.jcv.2012.03.008. View

13.

Ksiazek T, Erdman D, Goldsmith C, Zaki S, Peret T, Emery S . A novel coronavirus associated with severe acute respiratory syndrome. N Engl J Med. 2003; 348(20):1953-66. DOI: 10.1056/NEJMoa030781. View

14.

Niska R, Bhuiya F, Xu J . National Hospital Ambulatory Medical Care Survey: 2007 emergency department summary. Natl Health Stat Report. 2010; (26):1-31. View

15.

Allander T, Tammi M, Eriksson M, Bjerkner A, Tiveljung-Lindell A, Andersson B . Cloning of a human parvovirus by molecular screening of respiratory tract samples. Proc Natl Acad Sci U S A. 2005; 102(36):12891-6. PMC: 1200281. DOI: 10.1073/pnas.0504666102. View

16.

Lee A, Atkinson C, Manuel R, Clark D . Comparative evaluation of the QIAGEN QIAsymphony® SP system and bioMérieux NucliSens easyMAG automated extraction platforms in a clinical virology laboratory. J Clin Virol. 2011; 52(4):339-43. DOI: 10.1016/j.jcv.2011.08.016. View

17.

Kehl S, Kumar S . Utilization of nucleic acid amplification assays for the detection of respiratory viruses. Clin Lab Med. 2009; 29(4):661-71. PMC: 7131773. DOI: 10.1016/j.cll.2009.07.008. View

18.

Utokaparch S, Marchant D, Gosselink J, McDonough J, Thomas E, Hogg J . The relationship between respiratory viral loads and diagnosis in children presenting to a pediatric hospital emergency department. Pediatr Infect Dis J. 2010; 30(2):e18-23. DOI: 10.1097/INF.0b013e3181ff2fac. View

19.

Navarro-Mari J, Perez-Ruiz M . [Respiratory viruses: old and new. Review of diagnostic methods]. Enferm Infecc Microbiol Clin. 2024; 25:60-65. PMC: 7130279. DOI: 10.1157/13111839. View

20.

Hing E, Hall M, Ashman J, Xu J . National Hospital Ambulatory Medical Care Survey: 2007 outpatient department summary. Natl Health Stat Report. 2010; (28):1-32. View