Comparison of the NucliSens EasyMAG and Qiagen BioRobot 9604 Nucleic Acid Extraction Systems for Detection of RNA and DNA Respiratory Viruses in Nasopharyngeal Aspirate Samples

Overview

Journal J Clin Microbiol

Specialty Microbiology

Date 2008 May 9

PMID 18463206

Citations 25

Authors

Kwok Hung Chan

Wing Cheong Yam

Chiu Mei Pang

Kit Man Chan

Siu Yan Lam

Kam Fai Lo

Leo L M Poon

J S Malik Peiris

Affiliations

Soon will be listed here.

Abstract

The NucliSens easyMAG and BioRot 9604 automated nucleic acid extraction systems were evaluated and compared with the manual QIAamp (Qiagen) extraction method for their abilities to extract nucleic acid from nasopharyngeal aspirate samples for the detection of RNA and DNA respiratory viruses. The nucleic acids recovered by all three methods gave comparable sensitivities in PCR tests, and the three methods gave comparable viral loads. There was no evidence of residual PCR inhibitors and no evidence of PCR cross-contamination.

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