The Fidelity of Transcription: RPB1 (RPO21) Mutations That Increase Transcriptional Slippage in S. Cerevisiae

Overview

Journal J Biol Chem

Specialty Biochemistry

Date 2012 Dec 11

PMID 23223234

Citations 25

Authors

Jeffrey Strathern

Francisco Malagon

Jordan Irvin

Deanna Gotte

Brenda Shafer

Maria KIreeva

Lucyna Lubkowska

Ding Jun Jin

Mikhail Kashlev

Affiliations

Soon will be listed here.

Abstract

The fidelity of RNA synthesis depends on both accurate template-mediated nucleotide selection and proper maintenance of register between template and RNA. Loss of register, or transcriptional slippage, is particularly likely on homopolymeric runs in the template. Transcriptional slippage can alter the coding capacity of mRNAs and is used as a regulatory mechanism. Here we describe mutations in the largest subunit of Saccharomyces cerevisiae RNA polymerase II that substantially increase the level of transcriptional slippage. Alleles of RPB1 (RPO21) with elevated slippage rates were identified among 6-azauracil-sensitive mutants and were also isolated using a slippage-dependent reporter gene. Biochemical characterization of polymerase II isolated from these mutants confirms elevated levels of transcriptional slippage.

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