Transient Reversal of RNA Polymerase II Active Site Closing Controls Fidelity of Transcription Elongation

Overview

Journal Mol Cell

Publisher Cell Press

Specialty Cell Biology

Date 2008 Jun 10

PMID 18538654

Citations 117

Authors

Maria L Kireeva

Yuri A Nedialkov

Gina H Cremona

Yuri A Purtov

Lucyna Lubkowska

Francisco Malagon

Zachary F Burton

Jeffrey N Strathern

Mikhail Kashlev

Affiliations

Soon will be listed here.

Abstract

To study fidelity of RNA polymerase II (Pol II), we analyzed properties of the 6-azauracil-sensitive and TFIIS-dependent E1103G mutant of rbp1 (rpo21), the gene encoding the catalytic subunit of Pol II in Saccharomyces cerevisiae. Using an in vivo retrotransposition-based transcription fidelity assay, we observed that rpb1-E1103G causes a 3-fold increase in transcription errors. This mutant showed a 10-fold decrease in fidelity of transcription elongation in vitro. The mutation does not appear to significantly affect translocation state equilibrium of Pol II in a stalled elongation complex. Primarily, it promotes NTP sequestration in the polymerase active center. Furthermore, pre-steady-state analyses revealed that the E1103G mutation shifted the equilibrium between the closed and the open active center conformations toward the closed form. Thus, open conformation of the active center emerges as an intermediate essential for preincorporation fidelity control. Similar mechanisms may control fidelity of DNA-dependent DNA polymerases and RNA-dependent RNA polymerases.

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