Redox Regulation of Epidermal Growth Factor Receptor Signaling Through Cysteine Oxidation

Overview

Journal Biochemistry

Specialty Biochemistry

Date 2012 Nov 29

PMID 23186290

Citations 83

Authors

Thu H Truong

Kate S Carroll

Affiliations

Soon will be listed here.

Abstract

Epidermal growth factor receptor (EGFR) exemplifies the family of receptor tyrosine kinases that mediate numerous cellular processes, including growth, proliferation, and differentiation. Moreover, gene amplification and EGFR mutations have been identified in a number of human malignancies, making this receptor an important target for the development of anticancer drugs. In addition to ligand-dependent activation and concomitant tyrosine phosphorylation, EGFR stimulation results in the localized generation of H(2)O(2) by NADPH-dependent oxidases. In turn, H(2)O(2) functions as a secondary messenger to regulate intracellular signaling cascades, largely through the modification of specific cysteine residues within redox-sensitive protein targets, including Cys797 in the EGFR active site. In this review, we highlight recent advances in our understanding of the mechanisms that underlie redox regulation of EGFR signaling and how these discoveries may form the basis for the development of new therapeutic strategies for targeting this and other H(2)O(2)-modulated pathways.

Citing Articles

Protein phosphatase PP2Cα S-glutathionylation regulates cell migration.

Kukulage D, Samarasinghe K, Matarage Don N, Shivamadhu M, Shishikura K, Schiff W J Biol Chem. 2024; 300(10):107784.

PMID: 39303918 PMC: 11530597. DOI: 10.1016/j.jbc.2024.107784.

Reactive Oxygen Species Mechanisms that Regulate Protein-Protein Interactions in Cancer.

Iliadis S, Papanikolaou N Int J Mol Sci. 2024; 25(17).

PMID: 39273204 PMC: 11395503. DOI: 10.3390/ijms25179255.

Reactive oxygen species activate the Drosophila TNF receptor Wengen for damage-induced regeneration.

Esteban-Collado J, Fernandez-Manas M, Fernandez-Moreno M, Maeso I, Corominas M, Serras F EMBO J. 2024; 43(17):3604-3626.

PMID: 39020149 PMC: 11377715. DOI: 10.1038/s44318-024-00155-9.

Pro-Oxidant Auranofin and Glutathione-Depleting Combination Unveils Synergistic Lethality in Glioblastoma Cells with Aberrant Epidermal Growth Factor Receptor Expression.

Martinez-Jaramillo E, Jamali F, Abdalbari F, Abdulkarim B, Jean-Claude B, Telleria C Cancers (Basel). 2024; 16(13).

PMID: 39001381 PMC: 11240359. DOI: 10.3390/cancers16132319.

Metabolic profiling and combined therapeutic strategies unveil the cytotoxic potential of selenium-chrysin (SeChry) in NSCLC cells.

Mendes C, Lemos I, Hipolito A, Abreu B, Freitas-Dias C, Martins F Biosci Rep. 2024; 44(7).

PMID: 38990147 PMC: 11292474. DOI: 10.1042/BSR20240752.

References

Oh H, Jung H, Kim J, Bae Y . Phosphorylation of serine282 in NADPH oxidase activator 1 by Erk desensitizes EGF-induced ROS generation. Biochem Biophys Res Commun. 2010; 394(3):691-6. DOI: 10.1016/j.bbrc.2010.03.053. View

Tonks N . Protein tyrosine phosphatases: from genes, to function, to disease. Nat Rev Mol Cell Biol. 2006; 7(11):833-46. DOI: 10.1038/nrm2039. View

Wood Z, Schroder E, Harris J, Poole L . Structure, mechanism and regulation of peroxiredoxins. Trends Biochem Sci. 2003; 28(1):32-40. DOI: 10.1016/s0968-0004(02)00003-8. View

Truong T, Garcia F, Seo Y, Carroll K . Isotope-coded chemical reporter and acid-cleavable affinity reagents for monitoring protein sulfenic acids. Bioorg Med Chem Lett. 2011; 21(17):5015-20. DOI: 10.1016/j.bmcl.2011.04.115. View

Rostovtsev V, Green L, Fokin V, Sharpless K . A stepwise huisgen cycloaddition process: copper(I)-catalyzed regioselective "ligation" of azides and terminal alkynes. Angew Chem Int Ed Engl. 2002; 41(14):2596-9. DOI: 10.1002/1521-3773(20020715)41:14<2596::AID-ANIE2596>3.0.CO;2-4. View

Wang X, Martindale J, Liu Y, Holbrook N . The cellular response to oxidative stress: influences of mitogen-activated protein kinase signalling pathways on cell survival. Biochem J. 1998; 333 ( Pt 2):291-300. PMC: 1219585. DOI: 10.1042/bj3330291. View

Wu Y, Kwon K, Rhee S . Probing cellular protein targets of H2O2 with fluorescein-conjugated iodoacetamide and antibodies to fluorescein. FEBS Lett. 1998; 440(1-2):111-5. DOI: 10.1016/s0014-5793(98)01415-x. View

Benitez L, Allison W . The inactivation of the acyl phosphatase activity catalyzed by the sulfenic acid form of glyceraldehyde 3-phosphate dehydrogenase by dimedone and olefins. J Biol Chem. 1974; 249(19):6234-43. View

Hsu J, Hsieh Y, Tu C, OConnor D, Nick H, Silverman D . Catalytic properties of human manganese superoxide dismutase. J Biol Chem. 1996; 271(30):17687-91. DOI: 10.1074/jbc.271.30.17687. View

10.

McCord J, Fridovich I . Superoxide dismutase. An enzymic function for erythrocuprein (hemocuprein). J Biol Chem. 1969; 244(22):6049-55. View

11.

Yun C, Mengwasser K, Toms A, Woo M, Greulich H, Wong K . The T790M mutation in EGFR kinase causes drug resistance by increasing the affinity for ATP. Proc Natl Acad Sci U S A. 2008; 105(6):2070-5. PMC: 2538882. DOI: 10.1073/pnas.0709662105. View

12.

Aslan M, Ozben T . Oxidants in receptor tyrosine kinase signal transduction pathways. Antioxid Redox Signal. 2003; 5(6):781-8. DOI: 10.1089/152308603770380089. View

13.

Juarez J, Manuia M, Burnett M, Betancourt O, Boivin B, Shaw D . Superoxide dismutase 1 (SOD1) is essential for H2O2-mediated oxidation and inactivation of phosphatases in growth factor signaling. Proc Natl Acad Sci U S A. 2008; 105(20):7147-52. PMC: 2438219. DOI: 10.1073/pnas.0709451105. View

14.

Chen K, Kirber M, Xiao H, Yang Y, Keaney Jr J . Regulation of ROS signal transduction by NADPH oxidase 4 localization. J Cell Biol. 2008; 181(7):1129-39. PMC: 2442210. DOI: 10.1083/jcb.200709049. View

15.

Miller E, Dickinson B, Chang C . Aquaporin-3 mediates hydrogen peroxide uptake to regulate downstream intracellular signaling. Proc Natl Acad Sci U S A. 2010; 107(36):15681-6. PMC: 2936599. DOI: 10.1073/pnas.1005776107. View

16.

Lo Y, Cruz T . Involvement of reactive oxygen species in cytokine and growth factor induction of c-fos expression in chondrocytes. J Biol Chem. 1995; 270(20):11727-30. DOI: 10.1074/jbc.270.20.11727. View

17.

Giorgio M, Trinei M, Migliaccio E, Pelicci P . Hydrogen peroxide: a metabolic by-product or a common mediator of ageing signals?. Nat Rev Mol Cell Biol. 2007; 8(9):722-8. DOI: 10.1038/nrm2240. View

18.

Chen K, Vita J, Berk B, Keaney Jr J . c-Jun N-terminal kinase activation by hydrogen peroxide in endothelial cells involves SRC-dependent epidermal growth factor receptor transactivation. J Biol Chem. 2001; 276(19):16045-50. DOI: 10.1074/jbc.M011766200. View

19.

Murillo-Carretero M, Torroglosa A, Castro C, Villalobo A, Estrada C . S-Nitrosylation of the epidermal growth factor receptor: a regulatory mechanism of receptor tyrosine kinase activity. Free Radic Biol Med. 2008; 46(4):471-9. DOI: 10.1016/j.freeradbiomed.2008.10.048. View

20.

Lee S, Yang K, Kwon J, Lee C, Jeong W, Rhee S . Reversible inactivation of the tumor suppressor PTEN by H2O2. J Biol Chem. 2002; 277(23):20336-42. DOI: 10.1074/jbc.M111899200. View