Protein-engineered Injectable Hydrogel to Improve Retention of Transplanted Adipose-derived Stem Cells

Overview

Journal Adv Healthc Mater

Specialties Biomedical Engineering
Biotechnology

Date 2012 Nov 28

PMID 23184882

Citations 51

Authors

Andreina Parisi-Amon

Widya Mulyasasmita

Cindy Chung

Sarah C Heilshorn

Affiliations

Soon will be listed here.

Abstract

Improved retention of transplanted stem cells is achieved through minimally invasive delivery in MITCH, a mixing-induced two-component hydrogel that was engineered to possess shear-thinning and self-healing thixotropic properties. MITCH, an ideal injectable cell-delivery vehicle, supports 3D stem-cell culture, resulting in high cell viability and physiologically relevant cell morphology.

Citing Articles

The Optimization of Culture Conditions for Injectable Recombinant Collagen Hydrogel Preparation Using Machine Learning.

Li M, Zhao L, Ren Y, Zuo L, Shen Z, Wu J Gels. 2025; 11(2).

PMID: 39996684 PMC: 11855032. DOI: 10.3390/gels11020141.

Engineered Protein Hydrogels as Biomimetic Cellular Scaffolds.

Liu Y, Gilchrist A, Heilshorn S Adv Mater. 2024; 36(45):e2407794.

PMID: 39233559 PMC: 11573243. DOI: 10.1002/adma.202407794.

Injectable shear-thinning hydrogels promote oligodendrocyte progenitor cell survival and remyelination in the central nervous system.

Kumar Podder A, Mohamed M, Seidman R, Tseropoulos G, Polanco J, Lei P Sci Adv. 2024; 10(28):eadk9918.

PMID: 38996029 PMC: 11244542. DOI: 10.1126/sciadv.adk9918.

Chemical and Biological Engineering Strategies to Make and Modify Next-Generation Hydrogel Biomaterials.

Gharios R, Francis R, DeForest C Matter. 2024; 6(12):4195-4244.

PMID: 38313360 PMC: 10836217. DOI: 10.1016/j.matt.2023.10.012.

Effect of Flow Rate Modulation on Alginate Emulsification in Multistage Microfluidics.

Whulanza Y, Nathani R, Adimillenva K, Irwansyah R, Wahyu Nurhayati R, Utomo M Micromachines (Basel). 2023; 14(10).

PMID: 37893265 PMC: 10609249. DOI: 10.3390/mi14101828.

References

Webber M, Tongers J, Renault M, Roncalli J, Losordo D, Stupp S . Development of bioactive peptide amphiphiles for therapeutic cell delivery. Acta Biomater. 2009; 6(1):3-11. PMC: 2787676. DOI: 10.1016/j.actbio.2009.07.031. View

Aguado B, Mulyasasmita W, Su J, Lampe K, Heilshorn S . Improving viability of stem cells during syringe needle flow through the design of hydrogel cell carriers. Tissue Eng Part A. 2011; 18(7-8):806-15. PMC: 3313609. DOI: 10.1089/ten.TEA.2011.0391. View

Bliss T, Kelly S, Shah A, Foo W, Kohli P, Stokes C . Transplantation of hNT neurons into the ischemic cortex: cell survival and effect on sensorimotor behavior. J Neurosci Res. 2006; 83(6):1004-14. DOI: 10.1002/jnr.20800. View

de Almeida P, van Rappard J, Wu J . In vivo bioluminescence for tracking cell fate and function. Am J Physiol Heart Circ Physiol. 2011; 301(3):H663-71. PMC: 3191083. DOI: 10.1152/ajpheart.00337.2011. View

Contag C, Ross B . It's not just about anatomy: in vivo bioluminescence imaging as an eyepiece into biology. J Magn Reson Imaging. 2002; 16(4):378-87. DOI: 10.1002/jmri.10178. View

Sun N, Panetta N, Gupta D, Wilson K, Lee A, Jia F . Feeder-free derivation of induced pluripotent stem cells from adult human adipose stem cells. Proc Natl Acad Sci U S A. 2009; 106(37):15720-5. PMC: 2739869. DOI: 10.1073/pnas.0908450106. View

Lu H, Charati M, Kim I, Burdick J . Injectable shear-thinning hydrogels engineered with a self-assembling Dock-and-Lock mechanism. Biomaterials. 2011; 33(7):2145-53. DOI: 10.1016/j.biomaterials.2011.11.076. View

Serban M, Liu Y, Prestwich G . Effects of extracellular matrix analogues on primary human fibroblast behavior. Acta Biomater. 2007; 4(1):67-75. PMC: 2276336. DOI: 10.1016/j.actbio.2007.09.006. View

Garcia-Olmo D, Herreros D, Pascual I, Pascual J, Del-Valle E, Zorrilla J . Expanded adipose-derived stem cells for the treatment of complex perianal fistula: a phase II clinical trial. Dis Colon Rectum. 2009; 52(1):79-86. DOI: 10.1007/DCR.0b013e3181973487. View

10.

Singelyn J, DeQuach J, Seif-Naraghi S, Littlefield R, Schup-Magoffin P, Christman K . Naturally derived myocardial matrix as an injectable scaffold for cardiac tissue engineering. Biomaterials. 2009; 30(29):5409-16. PMC: 2728782. DOI: 10.1016/j.biomaterials.2009.06.045. View

11.

Kofidis T, Lebl D, Martinez E, Hoyt G, Tanaka M, Robbins R . Novel injectable bioartificial tissue facilitates targeted, less invasive, large-scale tissue restoration on the beating heart after myocardial injury. Circulation. 2005; 112(9 Suppl):I173-7. DOI: 10.1161/CIRCULATIONAHA.104.526178. View

12.

Cao F, Sadrzadeh Rafie A, Abilez O, Wang H, Blundo J, Pruitt B . In vivo imaging and evaluation of different biomatrices for improvement of stem cell survival. J Tissue Eng Regen Med. 2008; 1(6):465-8. PMC: 3657504. DOI: 10.1002/term.55. View

13.

Lu F, Mizuno H, Uysal C, Cai X, Ogawa R, Hyakusoku H . Improved viability of random pattern skin flaps through the use of adipose-derived stem cells. Plast Reconstr Surg. 2008; 121(1):50-58. DOI: 10.1097/01.prs.0000293876.10700.b8. View

14.

Tobita M, Orbay H, Mizuno H . Adipose-derived stem cells: current findings and future perspectives. Discov Med. 2011; 11(57):160-70. View

15.

Schneider J, Pochan D, Ozbas B, Rajagopal K, Pakstis L, Kretsinger J . Responsive hydrogels from the intramolecular folding and self-assembly of a designed peptide. J Am Chem Soc. 2002; 124(50):15030-7. DOI: 10.1021/ja027993g. View

16.

Bunnell B, Flaat M, Gagliardi C, Patel B, Ripoll C . Adipose-derived stem cells: isolation, expansion and differentiation. Methods. 2008; 45(2):115-20. PMC: 3668445. DOI: 10.1016/j.ymeth.2008.03.006. View

17.

Zhang M, Methot D, Poppa V, Fujio Y, Walsh K, Murry C . Cardiomyocyte grafting for cardiac repair: graft cell death and anti-death strategies. J Mol Cell Cardiol. 2001; 33(5):907-21. DOI: 10.1006/jmcc.2001.1367. View

18.

ZuK P, Zhu M, Ashjian P, De Ugarte D, Huang J, Mizuno H . Human adipose tissue is a source of multipotent stem cells. Mol Biol Cell. 2002; 13(12):4279-95. PMC: 138633. DOI: 10.1091/mbc.e02-02-0105. View

19.

Wong Po Foo C, Lee J, Mulyasasmita W, Parisi-Amon A, Heilshorn S . Two-component protein-engineered physical hydrogels for cell encapsulation. Proc Natl Acad Sci U S A. 2009; 106(52):22067-72. PMC: 2791665. DOI: 10.1073/pnas.0904851106. View

20.

Olsen B, Kornfield J, Tirrell D . Yielding Behavior in Injectable Hydrogels from Telechelic Proteins. Macromolecules. 2011; 43(21):9094-9099. PMC: 3017468. DOI: 10.1021/ma101434a. View