Completion of the Swine Genome Will Simplify the Production of Swine As a Large Animal Biomedical Model

Overview

Journal BMC Med Genomics

Publisher Biomed Central

Specialty Genetics

Date 2012 Nov 16

PMID 23151353

Citations 45

Authors

Eric M Walters

Eckhard Wolf

Jeffery J Whyte

Jiude Mao

Simone Renner

Hiroshi Nagashima

Eiji Kobayashi

Jianguo Zhao

Kevin D Wells

John K Critser

Lela K Riley

Randall S Prather

Affiliations

Soon will be listed here.

Abstract

Background: Anatomic and physiological similarities to the human make swine an excellent large animal model for human health and disease.

Methods: Cloning from a modified somatic cell, which can be determined in cells prior to making the animal, is the only method available for the production of targeted modifications in swine.

Results: Since some strains of swine are similar in size to humans, technologies that have been developed for swine can be readily adapted to humans and vice versa. Here the importance of swine as a biomedical model, current technologies to produce genetically enhanced swine, current biomedical models, and how the completion of the swine genome will promote swine as a biomedical model are discussed.

Conclusions: The completion of the swine genome will enhance the continued use and development of swine as models of human health, syndromes and conditions.

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References

Riordan J, Rommens J, Kerem B, Alon N, Rozmahel R, Grzelczak Z . Identification of the cystic fibrosis gene: cloning and characterization of complementary DNA. Science. 1989; 245(4922):1066-73. DOI: 10.1126/science.2475911. View

Rogers C, Hao Y, Rokhlina T, Samuel M, Stoltz D, Li Y . Production of CFTR-null and CFTR-DeltaF508 heterozygous pigs by adeno-associated virus-mediated gene targeting and somatic cell nuclear transfer. J Clin Invest. 2008; 118(4):1571-7. PMC: 2265103. DOI: 10.1172/JCI34773. View

Nauck M, Heimesaat M, Orskov C, Holst J, Ebert R, Creutzfeldt W . Preserved incretin activity of glucagon-like peptide 1 [7-36 amide] but not of synthetic human gastric inhibitory polypeptide in patients with type-2 diabetes mellitus. J Clin Invest. 1993; 91(1):301-7. PMC: 330027. DOI: 10.1172/JCI116186. View

Shaffer C . Incretin mimetics vie for slice of type 2 diabetes market. Nat Biotechnol. 2007; 25(3):263. DOI: 10.1038/nbt0307-263. View

Gordon J, Scangos G, Plotkin D, Barbosa J, Ruddle F . Genetic transformation of mouse embryos by microinjection of purified DNA. Proc Natl Acad Sci U S A. 1980; 77(12):7380-4. PMC: 350507. DOI: 10.1073/pnas.77.12.7380. View

Whitworth K, Zhao J, Spate L, Li R, Prather R . Scriptaid corrects gene expression of a few aberrantly reprogrammed transcripts in nuclear transfer pig blastocyst stage embryos. Cell Reprogram. 2011; 13(3):191-204. DOI: 10.1089/cell.2010.0087. View

Johnson Hamlet M, Yergeau D, Kuliyev E, Takeda M, Taira M, Kawakami K . Tol2 transposon-mediated transgenesis in Xenopus tropicalis. Genesis. 2006; 44(9):438-45. DOI: 10.1002/dvg.20234. View

Urnov F, Miller J, Lee Y, Beausejour C, Rock J, Augustus S . Highly efficient endogenous human gene correction using designed zinc-finger nucleases. Nature. 2005; 435(7042):646-51. DOI: 10.1038/nature03556. View

Du Z, Vincent-Naulleau S, Gilbert H, Vignoles F, Crechet F, Shimogiri T . Detection of novel quantitative trait loci for cutaneous melanoma by genome-wide scan in the MeLiM swine model. Int J Cancer. 2006; 120(2):303-20. DOI: 10.1002/ijc.22289. View

10.

Kawakami K, Shima A, Kawakami N . Identification of a functional transposase of the Tol2 element, an Ac-like element from the Japanese medaka fish, and its transposition in the zebrafish germ lineage. Proc Natl Acad Sci U S A. 2000; 97(21):11403-8. PMC: 17212. DOI: 10.1073/pnas.97.21.11403. View

11.

Guilbault C, Saeed Z, Downey G, Radzioch D . Cystic fibrosis mouse models. Am J Respir Cell Mol Biol. 2006; 36(1):1-7. DOI: 10.1165/rcmb.2006-0184TR. View

12.

Wright D, Thibodeau-Beganny S, Sander J, Winfrey R, Hirsh A, Eichtinger M . Standardized reagents and protocols for engineering zinc finger nucleases by modular assembly. Nat Protoc. 2007; 1(3):1637-52. DOI: 10.1038/nprot.2006.259. View

13.

Leahy P, Carmichael G, Rossomando E . Transcription from plasmid expression vectors is increased up to 14-fold when plasmids are transfected as concatemers. Nucleic Acids Res. 1997; 25(2):449-50. PMC: 146435. DOI: 10.1093/nar/25.2.449. View

14.

Kolber-Simonds D, Lai L, Watt S, Denaro M, Arn S, Augenstein M . Production of alpha-1,3-galactosyltransferase null pigs by means of nuclear transfer with fibroblasts bearing loss of heterozygosity mutations. Proc Natl Acad Sci U S A. 2004; 101(19):7335-40. PMC: 409919. DOI: 10.1073/pnas.0307819101. View

15.

Barash I, Ilan N, Kari R, Hurwitz D, Shani M . Co-integration of beta-lactoglobulin/human serum albumin hybrid genes with the entire beta-lactoglobulin gene or the matrix attachment region element: repression of human serum albumin and beta-lactoglobulin expression in the mammary gland and dual.... Mol Reprod Dev. 1996; 45(4):421-30. DOI: 10.1002/(SICI)1098-2795(199612)45:4<421::AID-MRD3>3.0.CO;2-T. View

16.

Harrison S, Guidolin A, Faast R, Crocker L, Giannakis C, dApice A . Efficient generation of alpha(1,3) galactosyltransferase knockout porcine fetal fibroblasts for nuclear transfer. Transgenic Res. 2002; 11(2):143-50. DOI: 10.1023/a:1015262108526. View

17.

Niemann H, Tian X, King W, Lee R . Epigenetic reprogramming in embryonic and foetal development upon somatic cell nuclear transfer cloning. Reproduction. 2008; 135(2):151-63. DOI: 10.1530/REP-07-0397. View

18.

Ramsoondar J, Machaty Z, Costa C, Williams B, Fodor W, Bondioli K . Production of alpha 1,3-galactosyltransferase-knockout cloned pigs expressing human alpha 1,2-fucosylosyltransferase. Biol Reprod. 2003; 69(2):437-45. DOI: 10.1095/biolreprod.102.014647. View

19.

Esteban M, Xu J, Yang J, Peng M, Qin D, Li W . Generation of induced pluripotent stem cell lines from Tibetan miniature pig. J Biol Chem. 2009; 284(26):17634-40. PMC: 2719402. DOI: 10.1074/jbc.M109.008938. View

20.

Watt S, Betthauser J, Augenstein M, Childs L, Mell G, Forsberg E . Direct and rapid modification of a porcine xenoantigen gene (GGTA1). Transplantation. 2006; 82(7):975-8. DOI: 10.1097/01.tp.0000229431.96906.33. View