Tracking Diphyodont Development in Miniature Pigs and

Overview

Journal Biol Open

Specialty Biology

Date 2019 Jan 27

PMID 30683673

Citations 4

Authors

Fu Wang

Guoqing Li

Zhifang Wu

Zhipeng Fan

Min Yang

Tingting Wu

Jinsong Wang

Chunmei Zhang

Songlin Wang

Affiliations

Soon will be listed here.

Abstract

Abnormalities of tooth number in humans, such as agenesis and supernumerary tooth formation, are closely related to diphyodont development. There is an increasing demand to understand the molecular and cellular mechanisms behind diphyodont development through the use of large animal models, since they are the most similar to the mechanism of human tooth development. However, attempting to study diphyodont development in large animals remains challenging due to large tooth size, prolonged growth stage and embryo manipulation. Here, we characterized the expression of possible genes for diphyodont development and odontogenesis of an organoid bud from single cells of tooth germs using strain (). Following this, we used a method of ectopic transplantation of tooth germs at cap stage to dynamically track diphyodont development of tooth germs in mouse subrenal capsules to overcome the restrictions in pig embryos. The results showed that pig tooth germ at cap stage could restore diphyodont development and maintain efficient long-term survival and growth in mouse subrenal capsules, which is suitable for future manipulation of large mammalian tooth development. Our pilot study provided an alternative for studying diphyodont development in large mammals, which will further promote the use of pig as a diphyodont model similar to humans for craniofacial development study.

Citing Articles

Spheroids and organoids: Their implications for oral and craniofacial tissue/organ regeneration.

Kagami H, Li X J Oral Biol Craniofac Res. 2024; 14(5):540-546.

PMID: 39092136 PMC: 11292544. DOI: 10.1016/j.jobcr.2024.07.002.

Morphogenesis of fungiform papillae in developing miniature pigs.

Wang L, Li J Heliyon. 2024; 10(3):e24953.

PMID: 38314265 PMC: 10837543. DOI: 10.1016/j.heliyon.2024.e24953.

Cocktail Formula and Application Prospects for Oral and Maxillofacial Organoids.

Ou M, Li Q, Ling X, Yao J, Mo X Tissue Eng Regen Med. 2022; 19(5):913-925.

PMID: 35612711 PMC: 9477993. DOI: 10.1007/s13770-022-00455-3.

Genome-Wide Characterization and Comparative Analyses of Simple Sequence Repeats among Four Miniature Pig Breeds.

Wang H, Fu Y, Gu P, Zhang Y, Tu W, Chao Z Animals (Basel). 2020; 10(10).

PMID: 33023098 PMC: 7600727. DOI: 10.3390/ani10101792.

References

Hu B, Nadiri A, Bopp-Kuchler S, Perrin-Schmitt F, Wang S, Lesot H . Dental epithelial histo-morphogenesis in the mouse: positional information versus cell history. Arch Oral Biol. 2005; 50(2):131-6. DOI: 10.1016/j.archoralbio.2004.09.007. View

Zhang Y, Chen Z, Song Y, Liu C, Chen Y . Making a tooth: growth factors, transcription factors, and stem cells. Cell Res. 2005; 15(5):301-16. DOI: 10.1038/sj.cr.7290299. View

Mazzeu J, Pardono E, Vianna-Morgante A, Richieri-Costa A, Kim C, Brunoni D . Clinical characterization of autosomal dominant and recessive variants of Robinow syndrome. Am J Med Genet A. 2007; 143(4):320-5. DOI: 10.1002/ajmg.a.31592. View

Wang S, Liu Y, Fang D, Shi S . The miniature pig: a useful large animal model for dental and orofacial research. Oral Dis. 2007; 13(6):530-7. DOI: 10.1111/j.1601-0825.2006.01337.x. View

Jarvinen E, Tummers M, Thesleff I . The role of the dental lamina in mammalian tooth replacement. J Exp Zool B Mol Dev Evol. 2009; 312B(4):281-91. DOI: 10.1002/jez.b.21275. View

Ikeda E, Morita R, Nakao K, Ishida K, Nakamura T, Takano-Yamamoto T . Fully functional bioengineered tooth replacement as an organ replacement therapy. Proc Natl Acad Sci U S A. 2009; 106(32):13475-80. PMC: 2720406. DOI: 10.1073/pnas.0902944106. View

Numakura C, Kitanaka S, Kato M, Ishikawa S, Hamamoto Y, Katsushima Y . Supernumerary impacted teeth in a patient with SOX2 anophthalmia syndrome. Am J Med Genet A. 2010; 152A(9):2355-9. DOI: 10.1002/ajmg.a.33556. View

Handrigan G, Leung K, Richman J . Identification of putative dental epithelial stem cells in a lizard with life-long tooth replacement. Development. 2010; 137(21):3545-9. DOI: 10.1242/dev.052415. View

Stembirek J, Buchtova M, Kral T, Matalova E, Lozanoff S, Misek I . Early morphogenesis of heterodont dentition in minipigs. Eur J Oral Sci. 2010; 118(6):547-58. DOI: 10.1111/j.1600-0722.2010.00772.x. View

10.

Richman J, Handrigan G . Reptilian tooth development. Genesis. 2011; 49(4):247-60. DOI: 10.1002/dvg.20721. View

11.

Deng W, Yang D, Zhao B, Ouyang Z, Song J, Fan N . Use of the 2A peptide for generation of multi-transgenic pigs through a single round of nuclear transfer. PLoS One. 2011; 6(5):e19986. PMC: 3094386. DOI: 10.1371/journal.pone.0019986. View

12.

Hauschild J, Petersen B, Santiago Y, Queisser A, Carnwath J, Lucas-Hahn A . Efficient generation of a biallelic knockout in pigs using zinc-finger nucleases. Proc Natl Acad Sci U S A. 2011; 108(29):12013-7. PMC: 3141985. DOI: 10.1073/pnas.1106422108. View

13.

Ekser B, Ezzelarab M, Hara H, van der Windt D, Wijkstrom M, Bottino R . Clinical xenotransplantation: the next medical revolution?. Lancet. 2011; 379(9816):672-83. DOI: 10.1016/S0140-6736(11)61091-X. View

14.

Buchtova M, Stembirek J, Glocova K, Matalova E, Tucker A . Early regression of the dental lamina underlies the development of diphyodont dentitions. J Dent Res. 2012; 91(5):491-8. DOI: 10.1177/0022034512442896. View

15.

Fluri D, Tonge P, Song H, Baptista R, Shakiba N, Shukla S . Derivation, expansion and differentiation of induced pluripotent stem cells in continuous suspension cultures. Nat Methods. 2012; 9(5):509-16. PMC: 4954777. DOI: 10.1038/nmeth.1939. View

16.

Juuri E, Saito K, Ahtiainen L, Seidel K, Tummers M, Hochedlinger K . Sox2+ stem cells contribute to all epithelial lineages of the tooth via Sfrp5+ progenitors. Dev Cell. 2012; 23(2):317-28. PMC: 3690347. DOI: 10.1016/j.devcel.2012.05.012. View

17.

Walters E, Wolf E, Whyte J, Mao J, Renner S, Nagashima H . Completion of the swine genome will simplify the production of swine as a large animal biomedical model. BMC Med Genomics. 2012; 5:55. PMC: 3499190. DOI: 10.1186/1755-8794-5-55. View

18.

Groenen M, Archibald A, Uenishi H, Tuggle C, Takeuchi Y, Rothschild M . Analyses of pig genomes provide insight into porcine demography and evolution. Nature. 2012; 491(7424):393-8. PMC: 3566564. DOI: 10.1038/nature11622. View

19.

Fan N, Chen J, Shang Z, Dou H, Ji G, Zou Q . Piglets cloned from induced pluripotent stem cells. Cell Res. 2012; 23(1):162-6. PMC: 3541650. DOI: 10.1038/cr.2012.176. View

20.

Sasai Y . Cytosystems dynamics in self-organization of tissue architecture. Nature. 2013; 493(7432):318-26. DOI: 10.1038/nature11859. View