Evaluation of Two Methods for Rapid Testing for Beta-lactamase Production in Bacteroides and Fusobacterium

Overview

Journal Eur J Clin Microbiol Infect Dis

Publisher Springer

Specialties Infectious Diseases
Microbiology

Date 1990 Jan 1

PMID 2303067

Citations 14

Authors

P C Appelbaum

S K Spangler

M R Jacobs

Affiliations

Soon will be listed here.

Abstract

A total of 978 strains of Bacteroides and Fusobacterium were tested for beta-lactamase production by a disk test (Cefinase) and a microtiter nitrocefin assay. In 83% of strains both tests were positive and in 14.8% both were negative. In 1.7% of strains the disk test was positive and the microtiter test negative, and in 0.4% the disk test negative but the microtiter test positive. The disk test was less discriminatory in detecting amoxicillin-resistant strains. The microtiter test was less sensitive than the disk test, but more discriminatory if results were read within 1 h for Fusobacterium spp., within 8 h for the Bacteroides fragilis group, and within 2 h for other Bacteroides spp. Neither test should be used clinically at present.

Citing Articles

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Use of a DNA microarray for simultaneous detection of antibiotic resistance genes among staphylococcal clinical isolates.

Zhu L, Zhang Z, Wang C, Yang H, Jiang D, Zhang Q J Clin Microbiol. 2007; 45(11):3514-21.

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Antimicrobial susceptibilities of clinical Desulfovibrio isolates.

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Comparative antianaerobic activity of BMS 284756.

Hoellman D, Kelly L, Jacobs M, Appelbaum P Antimicrob Agents Chemother. 2001; 45(2):589-92.

PMID: 11158759 PMC: 90331. DOI: 10.1128/AAC.45.2.589-592.2001.

Activities of gatifloxacin compared to those of seven other agents against anaerobic organisms.

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