The Transcription Factor MYB59 Regulates K/NO Translocation in the Arabidopsis Response to Low K Stress

Overview

Journal Plant Cell

Publisher Oxford University Press

Specialties Biology
Cell Biology

Date 2019 Feb 15

PMID 30760559

Citations 46

Authors

Xin-Qiao Du

Feng-Liu Wang

Hong Li

Si Jing

Miao Yu

Jigang Li

Wei-Hua Wu

Jorg Kudla

Yi Wang

Affiliations

Soon will be listed here.

Abstract

Potassium and nitrogen are essential nutrients for plant growth and development. Plants can sense potassium nitrate (K/NO ) levels in soils, and accordingly they adjust root-to-shoot K/NO transport to balance the distribution of these ions between roots and shoots. In this study, we show that the transcription factorMYB59 maintains this balance by regulating the transcription of the Arabidopsis () ()/ () in response to low K (LK) stress. The mutant showed a yellow-shoot sensitive phenotype when grown on LK medium. Both the transcript and protein levels of were remarkably reduced in the mutant. LK stress repressed transcript levels of both and The and mutants, as well as the double mutant, showed similar LK-sensitive phenotypes. Ion content analyses indicated that root-to-shoot K/NO transport was significantly reduced in these mutants under LK conditions. Moreover, chromatin immunoprecipitation and electrophoresis mobility shift assay assays confirmed that MYB59 bound directly to the promoter. Expression of in root vasculature driven by the promoter rescued the sensitive phenotype of both and mutants. Together, these data demonstrate that responds to LK stress and directs root-to-shoot K/NO transport by regulating the expression of in Arabidopsis roots.

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References

Gaymard F, Pilot G, Lacombe B, Bouchez D, Bruneau D, Boucherez J . Identification and disruption of a plant shaker-like outward channel involved in K+ release into the xylem sap. Cell. 1998; 94(5):647-55. DOI: 10.1016/s0092-8674(00)81606-2. View

Zhang X . Biogeochemistry: A plan for efficient use of nitrogen fertilizers. Nature. 2017; 543(7645):322-323. DOI: 10.1038/543322a. View

Jung C, Seo J, Han S, Koo Y, Kim C, Song S . Overexpression of AtMYB44 enhances stomatal closure to confer abiotic stress tolerance in transgenic Arabidopsis. Plant Physiol. 2007; 146(2):623-35. PMC: 2245844. DOI: 10.1104/pp.107.110981. View

Hickman R, Van Verk M, Van Dijken A, Pereira Mendes M, Vroegop-Vos I, Caarls L . Architecture and Dynamics of the Jasmonic Acid Gene Regulatory Network. Plant Cell. 2017; 29(9):2086-2105. PMC: 5635973. DOI: 10.1105/tpc.16.00958. View

Meng S, Peng J, He Y, Zhang G, Yi H, Fu Y . Arabidopsis NRT1.5 Mediates the Suppression of Nitrate Starvation-Induced Leaf Senescence by Modulating Foliar Potassium Level. Mol Plant. 2016; 9(3):461-470. DOI: 10.1016/j.molp.2015.12.015. View

Wang Y, Hsu P, Tsay Y . Uptake, allocation and signaling of nitrate. Trends Plant Sci. 2012; 17(8):458-67. DOI: 10.1016/j.tplants.2012.04.006. View

Prouse M, Campbell M . The interaction between MYB proteins and their target DNA binding sites. Biochim Biophys Acta. 2011; 1819(1):67-77. DOI: 10.1016/j.bbagrm.2011.10.010. View

Pettigrew W . Potassium influences on yield and quality production for maize, wheat, soybean and cotton. Physiol Plant. 2008; 133(4):670-81. DOI: 10.1111/j.1399-3054.2008.01073.x. View

Wang R, Tischner R, Gutierrez R, Hoffman M, Xing X, Chen M . Genomic analysis of the nitrate response using a nitrate reductase-null mutant of Arabidopsis. Plant Physiol. 2004; 136(1):2512-22. PMC: 523318. DOI: 10.1104/pp.104.044610. View

10.

Pyo Y, Gierth M, Schroeder J, Cho M . High-affinity K(+) transport in Arabidopsis: AtHAK5 and AKT1 are vital for seedling establishment and postgermination growth under low-potassium conditions. Plant Physiol. 2010; 153(2):863-75. PMC: 2879780. DOI: 10.1104/pp.110.154369. View

11.

Agarwal M, Hao Y, Kapoor A, Dong C, Fujii H, Zheng X . A R2R3 type MYB transcription factor is involved in the cold regulation of CBF genes and in acquired freezing tolerance. J Biol Chem. 2006; 281(49):37636-45. DOI: 10.1074/jbc.M605895200. View

12.

Wang Y, Wu W . Potassium transport and signaling in higher plants. Annu Rev Plant Biol. 2013; 64:451-76. DOI: 10.1146/annurev-arplant-050312-120153. View

13.

Hirsch R, Lewis B, Spalding E, Sussman M . A role for the AKT1 potassium channel in plant nutrition. Science. 1998; 280(5365):918-21. DOI: 10.1126/science.280.5365.918. View

14.

Nishida S, Kakei Y, Shimada Y, Fujiwara T . Genome-wide analysis of specific alterations in transcript structure and accumulation caused by nutrient deficiencies in Arabidopsis thaliana. Plant J. 2017; 91(4):741-753. DOI: 10.1111/tpj.13606. View

15.

Xing H, Dong L, Wang Z, Zhang H, Han C, Liu B . A CRISPR/Cas9 toolkit for multiplex genome editing in plants. BMC Plant Biol. 2014; 14:327. PMC: 4262988. DOI: 10.1186/s12870-014-0327-y. View

16.

Wang X, Niu Q, Teng C, Li C, Mu J, Chua N . Overexpression of PGA37/MYB118 and MYB115 promotes vegetative-to-embryonic transition in Arabidopsis. Cell Res. 2008; 19(2):224-35. DOI: 10.1038/cr.2008.276. View

17.

Li L, Yu X, Thompson A, Guo M, Yoshida S, Asami T . Arabidopsis MYB30 is a direct target of BES1 and cooperates with BES1 to regulate brassinosteroid-induced gene expression. Plant J. 2009; 58(2):275-86. PMC: 2814797. DOI: 10.1111/j.1365-313X.2008.03778.x. View

18.

Gierth M, Maser P, Schroeder J . The potassium transporter AtHAK5 functions in K(+) deprivation-induced high-affinity K(+) uptake and AKT1 K(+) channel contribution to K(+) uptake kinetics in Arabidopsis roots. Plant Physiol. 2005; 137(3):1105-14. PMC: 1065410. DOI: 10.1104/pp.104.057216. View

19.

Li J, Li X, Guo L, Lu F, Feng X, He K . A subgroup of MYB transcription factor genes undergoes highly conserved alternative splicing in Arabidopsis and rice. J Exp Bot. 2006; 57(6):1263-73. DOI: 10.1093/jxb/erj094. View

20.

Chen Y, Wang Y, Wu W . Membrane transporters for nitrogen, phosphate and potassium uptake in plants. J Integr Plant Biol. 2008; 50(7):835-48. DOI: 10.1111/j.1744-7909.2008.00707.x. View