Single-cell Expression Analysis of BMP15 and GDF9 in Mature Oocytes and BMPR2 in Cumulus Cells of Women with Polycystic Ovary Syndrome Undergoing Controlled Ovarian Hyperstimulation

Overview

Journal J Assist Reprod Genet

Publisher Springer

Specialties Genetics
Reproductive Medicine

Date 2012 Jul 25

PMID 22825968

Citations 16

Authors

Luciana Ochuiuto Teixeira de Resende

Alessandra Aparecida Vireque

Laura Ferreira Santana

Daniel Antunes Moreno

Ana Carolina Japur de Sa Rosa E Silva

Rui Alberto Ferriani

Carlos Alberto Scrideli

Rosana Maria Reis

Affiliations

Soon will be listed here.

Abstract

Purpose: To detect expression of bone morphogenetic protein 15 (BMP15) and growth differentiation factor 9 (GDF9) in oocytes, and their receptor type 2 receptor for BMPs (BMPR2) in cumulus cells in women with polycystic ovary syndrome (PCOS) undergoing in vitro fertilization (IVF), and determine if BMPR2, BMP15, and GDF9 expression correlate with hyperandrogenism in FF of PCOS patients.

Methods: Prospective case-control study. Eighteen MII-oocytes and their respective cumulus cells were obtained from 18 patients with PCOS, and 48 MII-oocytes and cumulus cells (CCs) from 35 controls, both subjected to controlled ovarian hyperstimulation (COH), and follicular fluid (FF) was collected from small (10-14 mm) and large (>18 mm) follicles. RNeasy Micro Kit (Qiagen) was used for RNA extraction and gene expression was quantified in each oocyte individually and in microdissected cumulus cells from cumulus-oocyte complexes retrieved from preovulatory follicles using qRT-PCR. Chemiluminescence and RIA assays were used for hormone assays.

Results: BMP15 and GDF9 expression per oocyte was higher among women with PCOS than the control group. A positive correlation was found between BMPR2 transcripts and hyperandrogenism in FF of PCOS patients. Progesterone values in FF were lower in the PCOS group.

Conclusion: We inferred that BMP15 and GDF9 transcript levels increase in mature PCOS oocytes after COH, and might inhibit the progesterone secretion by follicular cells in PCOS follicles, preventing premature luteinization in cumulus cells. BMPR2 expression in PCOS cumulus cells might be regulated by androgens.

Citing Articles

Serum GDF9 and BMP15 as potential markers of ovarian function in women with and without polycystic ovary syndrome.

Riepsamen A, Donoghoe M, Indran I, Hechtman L, Robertson D, Gilchrist R Clin Endocrinol (Oxf). 2022; 98(4):567-577.

PMID: 36372988 PMC: 10952143. DOI: 10.1111/cen.14851.

Concentrations of oocyte secreted GDF9 and BMP15 decrease with MII transition during human IVM.

Cadenas J, Pors S, Kumar A, Kalra B, Kristensen S, Andersen C Reprod Biol Endocrinol. 2022; 20(1):126.

PMID: 35986324 PMC: 9389727. DOI: 10.1186/s12958-022-01000-6.

Regulatory Role and Potential Importance of GDF-8 in Ovarian Reproductive Activity.

Zheng X, Zheng Y, Qin D, Yao Y, Zhang X, Zhao Y Front Endocrinol (Lausanne). 2022; 13:878069.

PMID: 35692411 PMC: 9178251. DOI: 10.3389/fendo.2022.878069.

Intrafollicular Concentrations of the Oocyte-secreted Factors GDF9 and BMP15 Vary Inversely in Polycystic Ovaries.

Kristensen S, Kumar A, Mamsen L, Kalra B, Pors S, Botkjaer J J Clin Endocrinol Metab. 2022; 107(8):e3374-e3383.

PMID: 35511085 PMC: 9282257. DOI: 10.1210/clinem/dgac272.

Compromised Cumulus-Oocyte Complex Matrix Organization and Expansion in Women with PCOS.

Patil K, Shinde G, Hinduja I, Mukherjee S Reprod Sci. 2021; 29(3):836-848.

PMID: 34748173 DOI: 10.1007/s43032-021-00775-0.

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