Structural and Comparative Analysis of the T Cell Receptor Gamma (TRG) Locus in Oryctolagus Cuniculus

Overview

Journal Immunogenetics

Specialties Allergy & Immunology
Genetics

Date 2012 Jul 10

PMID 22772779

Citations 11

Authors

Serafina Massari

Salvatrice Ciccarese

Rachele Antonacci

Affiliations

Soon will be listed here.

Abstract

In mammals, T cells develop along two discrete pathways characterized by expression of either the αβ or the γδT cell receptors. Human, mouse, and dog display a low peripheral blood γδ T cell percentage, while sheep accounts for a high proportion of γδ T lymphocytes. In all these species, the genomic organization of the T cell receptor gamma (TRG) locus is well known. To gain further insight into the evolutionary significance of the γδ T cell lineage, the present study has defined the genomic organization of the TRG locus in rabbit (Oryctolagus cuniculus), another mammalian γδ high species, as deduced from the genome assembly. The rabbit TRG locus spans about 70 kb and consists of ten TRGV, two TRGJ genes, and one TRGC gene located 5' to 3' in the locus. When we compared the rabbit sequence with the human, mouse, sheep, and dog counterparts, a higher identity with human as well as sheep with respect to mouse and dog was evident, providing that in the different mammalian species, the TRG locus appears to have evolved independently without any correlation with the γδ condition. The complete sequence of the rabbit TRG locus described here provides also a resource for supporting functional studies especially in the context of the γδ T cell function.

Citing Articles

Genomic and comparative analysis of the T cell receptor gamma locus in two species.

Massari S, Giannico F, Paolillo N, Pala A, Caputi Jambrenghi A, Antonacci R Front Immunol. 2023; 14:1264949.

PMID: 37781375 PMC: 10540303. DOI: 10.3389/fimmu.2023.1264949.

Characterization of the chicken T cell receptor γ repertoire by high-throughput sequencing.

Zhang T, Li Q, Li X, Kang L, Jiang Y, Sun Y BMC Genomics. 2021; 22(1):683.

PMID: 34548028 PMC: 8456604. DOI: 10.1186/s12864-021-07975-7.

Evolution of the T-Cell Receptor (TR) Loci in the Adaptive Immune Response: The Tale of the TRG Locus in Mammals.

Antonacci R, Massari S, Linguiti G, Caputi Jambrenghi A, Giannico F, Lefranc M Genes (Basel). 2020; 11(6).

PMID: 32517024 PMC: 7349638. DOI: 10.3390/genes11060624.

Topology and expressed repertoire of the Felis catus T cell receptor loci.

Radtanakatikanon A, Keller S, Darzentas N, Moore P, Folch G, Nguefack Ngoune V BMC Genomics. 2020; 21(1):20.

PMID: 31906850 PMC: 6945721. DOI: 10.1186/s12864-019-6431-5.

Divergent Evolution of TRC Genes in Mammalian Niche Adaptation.

Zhang Z, Mu Y, Shan L, Sun D, Guo W, Yu Z Front Immunol. 2019; 10:871.

PMID: 31068942 PMC: 6491686. DOI: 10.3389/fimmu.2019.00871.

References

Janeway Jr C, Jones B, Hayday A . Specificity and function of T cells bearing gamma delta receptors. Immunol Today. 1988; 9(3):73-6. DOI: 10.1016/0167-5699(88)91267-4. View

Miccoli M, Antonacci R, Vaccarelli G, Lanave C, Massari S, Cribiu E . Evolution of TRG clusters in cattle and sheep genomes as drawn from the structural analysis of the ovine TRG2@ locus. J Mol Evol. 2003; 57(1):52-62. DOI: 10.1007/s00239-002-2451-9. View

Lefranc M, Chuchana P, Dariavach P, Nguyen C, Huck S, Brockly F . Molecular mapping of the human T cell receptor gamma (TRG) genes and linkage of the variable and constant regions. Eur J Immunol. 1989; 19(6):989-94. DOI: 10.1002/eji.1830190606. View

Lefranc M, Pommie C, Kaas Q, Duprat E, Bosc N, Guiraudou D . IMGT unique numbering for immunoglobulin and T cell receptor constant domains and Ig superfamily C-like domains. Dev Comp Immunol. 2004; 29(3):185-203. DOI: 10.1016/j.dci.2004.07.003. View

Lefranc M, Pommie C, Ruiz M, Giudicelli V, Foulquier E, Truong L . IMGT unique numbering for immunoglobulin and T cell receptor variable domains and Ig superfamily V-like domains. Dev Comp Immunol. 2002; 27(1):55-77. DOI: 10.1016/s0145-305x(02)00039-3. View

Bininda-Emonds O, Cardillo M, Jones K, MacPhee R, Beck R, Grenyer R . The delayed rise of present-day mammals. Nature. 2007; 446(7135):507-12. DOI: 10.1038/nature05634. View

Vernooij B, Lenstra J, Wang K, Hood L . Organization of the murine T-cell receptor gamma locus. Genomics. 1993; 17(3):566-74. DOI: 10.1006/geno.1993.1373. View

Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S . MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol. 2011; 28(10):2731-9. PMC: 3203626. DOI: 10.1093/molbev/msr121. View

Hein W, Mackay C . Prominence of gamma delta T cells in the ruminant immune system. Immunol Today. 1991; 12(1):30-4. DOI: 10.1016/0167-5699(91)90109-7. View

10.

Lefranc M . From IMGT-ONTOLOGY CLASSIFICATION Axiom to IMGT standardized gene and allele nomenclature: for immunoglobulins (IG) and T cell receptors (TR). Cold Spring Harb Protoc. 2011; 2011(6):627-32. DOI: 10.1101/pdb.ip84. View

11.

Spencer D, Hsiang Y, Goldman J, Raulet D . Identification of a T-cell-specific transcriptional enhancer located 3' of C gamma 1 in the murine T-cell receptor gamma locus. Proc Natl Acad Sci U S A. 1991; 88(3):800-4. PMC: 50901. DOI: 10.1073/pnas.88.3.800. View

12.

Massari S, Bellahcene F, Vaccarelli G, Carelli G, Mineccia M, Lefranc M . The deduced structure of the T cell receptor gamma locus in Canis lupus familiaris. Mol Immunol. 2009; 46(13):2728-36. DOI: 10.1016/j.molimm.2009.05.008. View

13.

Schwartz S, Zhang Z, Frazer K, Smit A, Riemer C, Bouck J . PipMaker--a web server for aligning two genomic DNA sequences. Genome Res. 2000; 10(4):577-86. PMC: 310868. DOI: 10.1101/gr.10.4.577. View

14.

Giudicelli V, Chaume D, Lefranc M . IMGT/GENE-DB: a comprehensive database for human and mouse immunoglobulin and T cell receptor genes. Nucleic Acids Res. 2004; 33(Database issue):D256-61. PMC: 539964. DOI: 10.1093/nar/gki010. View

15.

Lefranc M . From IMGT-ONTOLOGY IDENTIFICATION axiom to IMGT standardized keywords: for immunoglobulins (IG), T cell receptors (TR), and conventional genes. Cold Spring Harb Protoc. 2011; 2011(6):604-13. DOI: 10.1101/pdb.ip82. View

16.

Massari S, Lipsi M, Vonghia G, Antonacci R, Ciccarese S . T-cell receptor TCRG1 and TCRG2 clusters map separately in two different regions of sheep chromosome 4. Chromosome Res. 1999; 6(5):419-20. DOI: 10.1023/a:1009245830804. View

17.

Faldyna M, Sinkora J, Knotigova P, Leva L, Toman M . Lymphatic organ development in dogs: major lymphocyte subsets and activity. Vet Immunol Immunopathol. 2005; 104(3-4):239-47. DOI: 10.1016/j.vetimm.2004.12.002. View

18.

Vaccarelli G, Miccoli M, Lanave C, Massari S, Cribiu E, Ciccarese S . Genomic organization of the sheep TRG1@ locus and comparative analyses of Bovidae and human variable genes. Gene. 2005; 357(2):103-14. DOI: 10.1016/j.gene.2005.05.033. View

19.

ISONO T, Kim C, Seto A . Sequence and diversity of rabbit T-cell receptor gamma chain genes. Immunogenetics. 1995; 41(5):295-300. DOI: 10.1007/BF00172154. View

20.

Hettmann T, Cohen A . Identification of a T cell-specific transcriptional enhancer 3' of the human T cell receptor gamma locus. Mol Immunol. 1994; 31(4):315-22. DOI: 10.1016/0161-5890(94)90129-5. View