Speeding Up Sequence Specific Assignment of IDPs

Overview

Journal J Biomol NMR

Publisher Springer

Specialties Molecular Biology
Nuclear Medicine

Date 2012 Jun 12

PMID 22684679

Citations 35

Authors

Wolfgang Bermel

Ivano Bertini

Isabella C Felli

Leonardo Gonnelli

Wiktor Kozminski

Alessandro Piai

Roberta Pierattelli

Jan Stanek

Affiliations

Soon will be listed here.

Abstract

The characterization of intrinsically disordered proteins (IDPs) by NMR spectroscopy is made difficult by the extensive spectral overlaps. To overcome the intrinsic low-resolution of the spectra the introduction of high-dimensionality experiments is essential. We present here a set of high-resolution experiments based on direct (13)C-detection which proved useful in the assignment of α-synuclein, a paradigmatic IDP. In particular, we describe the implementation of 4D HCBCACON, HCCCON, HCBCANCO, 4/5D HNCACON and HNCANCO and 3/4D HCANCACO experiments, specifically tailored for spin system identification and backbone resonances sequential assignment. The use of non-uniform-sampling in the indirect dimension and of the H-flip approach to achieve longitudinal relaxation enhancement rendered the experiments very practical.

Citing Articles

Optimal C NMR investigation of intrinsically disordered proteins at 1.2 GHz.

Schiavina M, Bracaglia L, Rodella M, Kummerle R, Konrat R, Felli I Nat Protoc. 2023; 19(2):406-440.

PMID: 38087081 DOI: 10.1038/s41596-023-00921-9.

N-Detected TROSY NMR experiments to study large disordered proteins in high-field magnets.

Dubey A, Viennet T, Chhabra S, Takeuchi K, Seo H, Bermel W Chem Commun (Camb). 2022; 58(68):9512-9515.

PMID: 35920752 PMC: 9578535. DOI: 10.1039/d2cc02005j.

C Direct Detected NMR for Challenging Systems.

Felli I, Pierattelli R Chem Rev. 2022; 122(10):9468-9496.

PMID: 35025504 PMC: 9136920. DOI: 10.1021/acs.chemrev.1c00871.

Unambiguous Tracking of Protein Phosphorylation by Fast High-Resolution FOSY NMR*.

Lesovoy D, Georgoulia P, Diercks T, Matecko-Burmann I, Burmann B, Bocharov E Angew Chem Int Ed Engl. 2021; 60(44):23540-23544.

PMID: 34143912 PMC: 8596425. DOI: 10.1002/anie.202102758.

HACANCOi: a new H-detected experiment for backbone resonance assignment of intrinsically disordered proteins.

Karjalainen M, Tossavainen H, Hellman M, Permi P J Biomol NMR. 2020; 74(12):741-752.

PMID: 33118136 PMC: 7701164. DOI: 10.1007/s10858-020-00347-5.

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