» Articles » PMID: 22679495

Transcriptome Kinetics of Circulating Neutrophils During Human Experimental Endotoxemia

Overview
Journal PLoS One
Date 2012 Jun 9
PMID 22679495
Citations 23
Authors
Affiliations
Soon will be listed here.
Abstract

Polymorphonuclear cells (neutrophils) play an important role in the systemic inflammatory response syndrome and the development of sepsis. These cells are essential for the defense against microorganisms, but may also cause tissue damage. Therefore, neutrophil numbers and activity are considered to be tightly regulated. Previous studies have investigated gene transcription during experimental endotoxemia in whole blood and peripheral blood mononuclear cells. However, the gene transcription response of the circulating pool of neutrophils to systemic inflammatory stimulation in vivo is currently unclear. We examined neutrophil gene transcription kinetics in healthy human subjects (n = 4) administered a single dose of endotoxin (LPS, 2 ng/kg iv). In addition, freshly isolated neutrophils were stimulated ex vivo with LPS, TNFα, G-CSF and GM-CSF to identify stimulus-specific gene transcription responses. Whole transcriptome microarray analysis of circulating neutrophils at 2, 4 and 6 hours after LPS infusion revealed activation of inflammatory networks which are involved in signaling of TNFα and IL-1α and IL-1β. The transcriptome profile of inflammatory activated neutrophils in vivo reflects extended survival and regulation of inflammatory responses. These changes in neutrophil transcriptome suggest a combination of early activation of circulating neutrophils by TNFα and G-CSF and a mobilization of young neutrophils from the bone marrow.

Citing Articles

Expression of proBDNF/p75 in peripheral blood lymphocytes of patients with sepsis and its impact on lymphocyte differentiation.

Wang S, Zeng Q, Gao H, Gao S, Dai R, Hu Z Zhong Nan Da Xue Xue Bao Yi Xue Ban. 2024; 48(11):1629-1638.

PMID: 38432853 PMC: 10929956. DOI: 10.11817/j.issn.1672-7347.2023.230179.


The association and prognostic value of systemic inflammatory response index with short and long-term mortality in patients with sepsis.

Ru S, Luo Y Medicine (Baltimore). 2023; 102(29):e33967.

PMID: 37478261 PMC: 10662841. DOI: 10.1097/MD.0000000000033967.


Identification of a forkhead box protein transcriptional network induced in human neutrophils in response to inflammatory stimuli.

Ismailova A, Salehi-Tabar R, Dimitrov V, Memari B, Barbier C, White J Front Immunol. 2023; 14:1123344.

PMID: 36756115 PMC: 9900176. DOI: 10.3389/fimmu.2023.1123344.


Aged versus fresh autologous platelet transfusion in a two-hit healthy volunteer model of transfusion-related acute lung injury.

van Baarle F, de Bruin S, Bulle E, van Mourik N, Lim E, Tuip-de Boer A Transfusion. 2022; 62(12):2490-2501.

PMID: 36300793 PMC: 10092071. DOI: 10.1111/trf.17157.


Immune System Disequilibrium-Neutrophils, Their Extracellular Traps, and COVID-19-Induced Sepsis.

Keane C, Coalter M, Martin-Loeches I Front Med (Lausanne). 2021; 8:711397.

PMID: 34485339 PMC: 8416266. DOI: 10.3389/fmed.2021.711397.


References
1.
Levy M, Fink M, Marshall J, Abraham E, Angus D, Cook D . 2001 SCCM/ESICM/ACCP/ATS/SIS International Sepsis Definitions Conference. Crit Care Med. 2003; 31(4):1250-6. DOI: 10.1097/01.CCM.0000050454.01978.3B. View

2.
Paunel-Gorgulu A, Flohe S, Scholz M, Windolf J, Logters T . Increased serum soluble Fas after major trauma is associated with delayed neutrophil apoptosis and development of sepsis. Crit Care. 2011; 15(1):R20. PMC: 3222054. DOI: 10.1186/cc9965. View

3.
Cowland J, Borregaard N . The individual regulation of granule protein mRNA levels during neutrophil maturation explains the heterogeneity of neutrophil granules. J Leukoc Biol. 1999; 66(6):989-95. DOI: 10.1002/jlb.66.6.989. View

4.
Hayashi F, Means T, Luster A . Toll-like receptors stimulate human neutrophil function. Blood. 2003; 102(7):2660-9. DOI: 10.1182/blood-2003-04-1078. View

5.
Tang B, McLean A, Dawes I, Huang S, Lin R . The use of gene-expression profiling to identify candidate genes in human sepsis. Am J Respir Crit Care Med. 2007; 176(7):676-84. DOI: 10.1164/rccm.200612-1819OC. View