Effect of Denervation on the Expression of Glycogen Phosphorylase in Mouse Skeletal Muscle

Overview

Journal Biochem J

Specialty Biochemistry

Date 1990 Nov 15

PMID 2264827

Citations 7

Authors

D M Leyland

P C Turner

R J Beynon

Affiliations

Soon will be listed here.

Abstract

After sciatectomy of the left hind-limb of C57BL/J mice, a denervation-induced muscular atrophy ensued and was accompanied by a decrease in the specific activity of glycogen phosphorylase to approx. 25% of control values. The cofactor of phosphorylase, pyridoxal 5'-phosphate, was used as a specific label in the determination of the degradation rate of the enzyme following nerve section. After a delay of 3-4 days, phosphorylase was degraded approx, twice as rapidly in the denervated gastrocnemius (0.20 day-1) as in the control muscle (0.12 day-1). The effect of denervation on phosphorylase mRNA was measured by quantitative Northern-blot analysis using a rat skeletal-muscle phosphorylase cDNA probe. After an initial rapid decline, phosphorylase mRNA levels stabilized in denervated muscle at 50% of the value measured in the contralateral control muscle.

Citing Articles

Nerve-dependent factors regulating transcript levels of glycogen phosphorylase in skeletal muscle.

Matthews C, Carlsen R, Froman B, Tait R, Gorin F Cell Mol Neurobiol. 1998; 18(3):319-38.

PMID: 9590562 PMC: 11560181. DOI: 10.1023/a:1022553115779.

Neural regulation of the formation of skeletal muscle phosphorylase kinase holoenzyme in adult and developing rat muscle.

Ng D, Carlsen R, Walsh D Biochem J. 1997; 325 ( Pt 3):793-800.

PMID: 9271102 PMC: 1218625. DOI: 10.1042/bj3250793.

Measurement of the turnover of glycogen phosphorylase by GC/MS using stable isotope derivatives of pyridoxine (vitamin B6).

Beynon R, Leyland D, Evershed R, EDWARDS R, Coburn S Biochem J. 1996; 317 ( Pt 2):613-9.

PMID: 8713093 PMC: 1217530. DOI: 10.1042/bj3170613.

Id-1 as a possible transcriptional mediator of muscle disuse atrophy.

Gundersen K, Merlie J Proc Natl Acad Sci U S A. 1994; 91(9):3647-51.

PMID: 8170962 PMC: 43638. DOI: 10.1073/pnas.91.9.3647.

Effect of vitamin B6 deficiency on the expression of glycogen phosphorylase mRNA in rat liver and skeletal muscle.

Oka T, Komori N, Kuwahata M, Suzuki I, Okada M, Natori Y Experientia. 1994; 50(2):127-9.

PMID: 8125170 DOI: 10.1007/BF01984949.

References

GOLDBERG A . Protein turnover in skeletal muscle. II. Effects of denervation and cortisone on protein catabolism in skeletal muscle. J Biol Chem. 1969; 244(12):3223-9. View

Turner L, Manchester K . Effects of denervation on the glycogen content and on the activities of enzymes of glucose and glycogen metabolism in rat diaphragm muscle. Biochem J. 1972; 128(4):789-801. PMC: 1173899. DOI: 10.1042/bj1280789. View

Studier F . Analysis of bacteriophage T7 early RNAs and proteins on slab gels. J Mol Biol. 1973; 79(2):237-48. DOI: 10.1016/0022-2836(73)90003-x. View

Goldspink D . The effects of denervation on protein turnover of rat skeletal muscle. Biochem J. 1976; 156(1):71-80. PMC: 1163718. DOI: 10.1042/bj1560071. View

Carney I, Beynon R, Kay J, Birket N . A semicontinuous assay for glycogen phosphorylase. Anal Biochem. 1978; 85(1):321-4. DOI: 10.1016/0003-2697(78)90309-3. View

Carney I, Beynon R, Kay J, Birket N . The susceptibility of muscle phosphorylases a and b to digestion by a neutral proteinase from rat intestinal muscle. Comparison with the effects produced by pancreatic trypsin and chymotrypsin. Biochem J. 1978; 175(1):105-13. PMC: 1186045. DOI: 10.1042/bj1750105. View

Towbin H, Staehelin T, Gordon J . Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci U S A. 1979; 76(9):4350-4. PMC: 411572. DOI: 10.1073/pnas.76.9.4350. View

Shackelford J, Lebherz H . Effect of denervation on the levels and rates of synthesis of specific enzymes in "fast-twitch" (breast) muscle fibers of the chicken. J Biol Chem. 1981; 256(12):6423-9. View

Joffe M, Savage N, Isaacs H . Increased muscle calcium. A possible cause of mitochondrial dysfunction and cellular necrosis in denervated rat skeletal muscle. Biochem J. 1981; 196(3):663-7. PMC: 1163083. DOI: 10.1042/bj1960663. View

10.

Rodemann H, GOLDBERG A . Arachidonic acid, prostaglandin E2 and F2 alpha influence rates of protein turnover in skeletal and cardiac muscle. J Biol Chem. 1982; 257(4):1632-8. View

11.

Cidlowski J, Kittler J, THANASSI J . Preparation, characterization, and use of monoclonal antibodies to vitamin B6. J Biol Chem. 1983; 258(4):2689-96. View

12.

Feinberg A, Vogelstein B . A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Anal Biochem. 1983; 132(1):6-13. DOI: 10.1016/0003-2697(83)90418-9. View

13.

Lebherz H . Content and synthesis of glycolytic enzymes in normal, denervated, and dystrophic skeletal muscle fibers. Int J Biochem. 1984; 16(12):1201-5. DOI: 10.1016/0020-711x(84)90217-9. View

14.

Butler P, Cookson E, Beynon R . Accelerated degradation of glycogen phosphorylase in denervated and dystrophic mouse skeletal muscle. Biosci Rep. 1985; 5(7):567-72. DOI: 10.1007/BF01117069. View

15.

Hwang P, See Y, Vincentini A, Powers M, Fletterick R, Crerar M . Comparative sequence analysis of rat, rabbit, and human muscle glycogen phosphorylase cDNAs. Eur J Biochem. 1985; 152(2):267-74. DOI: 10.1111/j.1432-1033.1985.tb09193.x. View

16.

Butler P, Cookson E, Beynon R . The turnover of skeletal muscle glycogen phosphorylase studied using the cofactor, pyridoxal phosphate, as a specific label. Biochim Biophys Acta. 1985; 847(3):316-23. DOI: 10.1016/0167-4889(85)90037-0. View

17.

Leader D, Gall I, Campbell P, Frischauf A . Isolation and characterization of cDNA clones from mouse skeletal muscle actin mRNA. DNA. 1986; 5(3):235-8. DOI: 10.1089/dna.1986.5.235. View

18.

TURINSKY J . Phospholipids, prostaglandin E2, and proteolysis in denervated muscle. Am J Physiol. 1986; 251(1 Pt 2):R165-73. DOI: 10.1152/ajpregu.1986.251.1.R165. View

19.

Leberer E, Pette D . Neural regulation of parvalbumin expression in mammalian skeletal muscle. Biochem J. 1986; 235(1):67-73. PMC: 1146649. DOI: 10.1042/bj2350067. View

20.

Henriksson J, Chi M, Hintz C, Young D, Kaiser K, Salmons S . Chronic stimulation of mammalian muscle: changes in enzymes of six metabolic pathways. Am J Physiol. 1986; 251(4 Pt 1):C614-32. DOI: 10.1152/ajpcell.1986.251.4.C614. View