Effect of Vitamin B6 Deficiency on the Expression of Glycogen Phosphorylase MRNA in Rat Liver and Skeletal Muscle

Overview

Journal Experientia

Specialty Science

Date 1994 Feb 15

PMID 8125170

Citations 2

Authors

T Oka

N Komori

M Kuwahata

I Suzuki

M Okada

Y Natori

Affiliations

Soon will be listed here.

Abstract

The effect of vitamin B6 deficiency on the expression of glycogen phosphorylase mRNA in rat liver and skeletal muscle was investigated. The level of phosphorylase mRNA in the muscle of vitamin B6-deficient rats was reduced to 40% of that in the control rats. By contrast, the phosphorylase mRNA level was increased 5-fold in the liver of the deficient animals. It was also found that the expression of the beta-actin gene, generally regarded as a 'housekeeping' gene, was unaffected by B6 deficiency in the muscle but was enhanced in the liver of the deficient animals. These observations suggest that vitamin B6 may modulate the transcriptional activation of the phosphorylase gene in a tissue-specific manner.

Citing Articles

Liver-specific increase of UTP and UDP-sugar concentrations in rats induced by dietary vitamin B6-deficiency and its relation to complex N-glycan structures of liver membrane-proteins.

Renner A, Rieger K, Grunow D, Zimmermann-Kordmann M, Gohlke M, Reutter W Glycoconj J. 2007; 24(9):531-41.

PMID: 17577663 DOI: 10.1007/s10719-007-9048-x.

Vitamin B6 modulates expression of albumin gene by inactivating tissue-specific DNA-binding protein in rat liver.

Oka T, Komori N, Kuwahata M, Okada M, Natori Y Biochem J. 1995; 309 ( Pt 1):243-8.

PMID: 7619063 PMC: 1135826. DOI: 10.1042/bj3090243.

References

Sato A, Nagai M, Tagami A, Natori Y, Okada M . Effect of pyridoxine-deficiency on degradation of cytosolic aspartate aminotransferase in rat liver lysosomes. J Nutr Sci Vitaminol (Tokyo). 1991; 37(4):419-24. DOI: 10.3177/jnsv.37.419. View

Okada M, Suzuki K . Amino acid metabolism in rats fed a high protein diet without pyridoxine. J Nutr. 1974; 104(3):287-93. DOI: 10.1093/jn/104.3.287. View

Leyland D, Turner P, Beynon R . Effect of denervation on the expression of glycogen phosphorylase in mouse skeletal muscle. Biochem J. 1990; 272(1):231-7. PMC: 1149681. DOI: 10.1042/bj2720231. View

Baranowski T, Illingworth B, Brown D, CORI C . The isolation of pyridoxal-5-phosphate from crystalline muscle phosphorylase. Biochim Biophys Acta. 1957; 25(1):16-21. DOI: 10.1016/0006-3002(57)90410-9. View

Fletterick R, MADSEN N . The structures and related functions of phosphorylase a. Annu Rev Biochem. 1980; 49:31-61. DOI: 10.1146/annurev.bi.49.070180.000335. View

Allgood V, Cidlowski J . Vitamin B6 modulates transcriptional activation by multiple members of the steroid hormone receptor superfamily. J Biol Chem. 1992; 267(6):3819-24. View

Okada M, Ishikawa K, Watanabe K . Effect of vitamin B6 deficiency on glycogen metabolism in the skeletal muscle, heart, and liver of rats. J Nutr Sci Vitaminol (Tokyo). 1991; 37(4):349-57. DOI: 10.3177/jnsv.37.349. View

Davis C, Schliselfeld L, Wolf D, Leavitt C, KREBS E . Interrelationships among glycogen phosphorylase isozymes. J Biol Chem. 1967; 242(20):4824-33. View

Shibuya M, Okada M . Effect of pyridoxine-deficiency on the turnover of aspartate aminotransferase isozymes in rat liver. J Biochem. 1986; 99(3):939-44. DOI: 10.1093/oxfordjournals.jbchem.a135556. View

10.

Dombradi V . Structural aspects of the catalytic and regulatory function of glycogen phosphorylase. Int J Biochem. 1981; 13(2):125-39. DOI: 10.1016/0020-711x(81)90147-6. View

11.

MacDonald R, Swift G, Przybyla A, Chirgwin J . Isolation of RNA using guanidinium salts. Methods Enzymol. 1987; 152:219-27. DOI: 10.1016/0076-6879(87)52023-7. View

12.

HENION W, SUTHERLAND E . Immunological differences of phosphorylases. J Biol Chem. 1957; 224(1):477-88. View

13.

Allgood V, Cidlowski J . Vitamin B6 influences glucocorticoid receptor-dependent gene expression. J Biol Chem. 1990; 265(21):12424-33. View

14.

Osawa S, Chiu R, McDonough A, Miller Jr T, Johnson G . Isolation of partial cDNAs for rat liver and muscle glycogen phosphorylase isozymes. FEBS Lett. 1986; 202(2):282-8. DOI: 10.1016/0014-5793(86)80702-5. View

15.

Oka T, Sasakawa T, Komori N, Miyamoto K, Suzuki I, Sassa T . Developmental changes in the expression of HMG 2a protein. FEBS Lett. 1993; 316(1):20-2. DOI: 10.1016/0014-5793(93)81728-i. View

16.

Oka T, Komori N, Kuwahata M, Sassa T, Suzuki I, Okada M . Vitamin B6 deficiency causes activation of RNA polymerase and general enhancement of gene expression in rat liver. FEBS Lett. 1993; 331(1-2):162-4. DOI: 10.1016/0014-5793(93)80318-o. View

17.

KENT A, KREBS E, FISCHER E . Properties of crystalline phosphorylase b. J Biol Chem. 1958; 232(1):549-58. View