Cloning, Functional Characterization and Genomic Organization of 1,8-cineole Synthases from Lavandula

Overview

Journal Plant Mol Biol

Date 2012 May 18

PMID 22592779

Citations 27

Authors

Zerihun A Demissie

Monica A Cella

Lukman S Sarker

Travis J Thompson

Mark R Rheault

Soheil S Mahmoud

Affiliations

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Abstract

Several members of the genus Lavandula produce valuable essential oils (EOs) that are primarily constituted of the low molecular weight isoprenoids, particularly monoterpenes. We isolated over 8,000 ESTs from the glandular trichomes of L. x intermedia flowers (where bulk of the EO is synthesized) to facilitate the discovery of genes that control the biosynthesis of EO constituents. The expression profile of these ESTs in L. x intermedia and its parents L. angustifolia and L. latifolia was established using microarrays. The resulting data highlighted a differentially expressed, previously uncharacterized cDNA with strong homology to known 1,8-cineole synthase (CINS) genes. The ORF, excluding the transit peptide, of this cDNA was expressed in E. coli, purified by Ni-NTA agarose affinity chromatography and functionally characterized in vitro. The ca. 63 kDa bacterially produced recombinant protein, designated L. x intermedia CINS (LiCINS), converted geranyl diphosphate (the linear monoterpene precursor) primarily to 1,8-cineole with K ( m ) and k ( cat ) values of 5.75 μM and 8.8 × 10(-3) s(-1), respectively. The genomic DNA of CINS in the studied Lavandula species had identical exon-intron architecture and coding sequences, except for a single polymorphic nucleotide in the L. angustifolia ortholog which did not alter protein function. Additional nucleotide variations restricted to L. angustifolia introns were also observed, suggesting that LiCINS was most likely inherited from L. latifolia. The LiCINS mRNA levels paralleled the 1,8-cineole content in mature flowers of the three lavender species, and in developmental stages of L. x intermedia inflorescence indicating that the production of 1,8 cineole in Lavandula is most likely controlled through transcriptional regulation of LiCINS.

Citing Articles

Metabolic engineering of terpene metabolism in lavender.

Oseni O, Sajaditabar R, Mahmoud S Beni Suef Univ J Basic Appl Sci. 2024; 13(1):67.

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Terpene produced by coexpression of the TPS and P450 genes from Lavandula angustifolia protects plants from herbivore attacks during budding stages.

Ling Z, Li J, Dong Y, Zhang W, Bai H, Li S BMC Plant Biol. 2023; 23(1):477.

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Decoupling subgenomes within hybrid lavandin provide new insights into speciation and monoterpenoid diversification of Lavandula.

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Lavandula Species, Their Bioactive Phytochemicals, and Their Biosynthetic Regulation.

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