Metabolites of Lactobacillus Plantarum 2142 Prevent Oxidative Stress-induced Overexpression of Proinflammatory Cytokines in IPEC-J2 Cell Line

Overview

Journal Inflammation

Specialties Allergy & Immunology
Pathology

Date 2012 Apr 6

PMID 22476971

Citations 21

Authors

Erzsebet Paszti-Gere

Krisztina Szeker

Edina Csibrik-Nemeth

Rita Csizinszky

Andras Marosi

Orsolya Palocz

Orsolya Farkas

Peter Galfi

Affiliations

Soon will be listed here.

Abstract

Probiotics have already proven beneficial effects in the treatment of several intestinal infections, but the underlying mechanisms how the probiotics can affect responses of porcine IPEC-J2 enterocytes to oxidative stress remained to be elucidated. The immunomodulatory effect of five bacterial strains (Lactobacillus plantarum 2142, Lactobacillus casei Shirota, Bifidobacterium animalis subsp. lactis BB-12, Bacillus amyloliquefaciens CECT 5940 and Enterococcus faecium CECT 4515) on 1 mM peroxide-triggered upregulation of interleukin (IL)-8 and tumor necrosis factor alpha (TNF-α) level was screened by q RT-PCR. Our data revealed that spent culture supernatant (SCS) of L. plantarum 2142 had significant lowering effect on IL-8 and TNF-α level with concomitant promoting activity on protective Hsp70 gene expression. According to our results, lactic acid (racemic, D: - and L: -lactic acid) and acetic acid produced by lactobacilli had no protective effect in quenching upregulation of proinflammatory cytokines. Furthermore, L. plantarum 2142-specific supernatant peptides were detected by gel electrophoresis and capillary zone electrophoresis.

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