Fast Optically Sectioned Fluorescence HiLo Endomicroscopy

Overview

Journal J Biomed Opt

Specialties Biomedical Engineering
Ophthalmology

Date 2012 Apr 3

PMID 22463023

Citations 34

Authors

Tim N Ford

Daryl Lim

Jerome Mertz

Affiliations

Soon will be listed here.

Abstract

We describe a nonscanning, fiber bundle endomicroscope that performs optically sectioned fluorescence imaging with fast frame rates and real-time processing. Our sectioning technique is based on HiLo imaging, wherein two widefield images are acquired under uniform and structured illumination and numerically processed to reject out-of-focus background. This work is an improvement upon an earlier demonstration of widefield optical sectioning through a flexible fiber bundle. The improved device features lateral and axial resolutions of 2.6 and 17 μm, respectively, a net frame rate of 9.5 Hz obtained by real-time image processing with a graphics processing unit (GPU) and significantly reduced motion artifacts obtained by the use of a double-shutter camera. We demonstrate the performance of our system with optically sectioned images and videos of a fluorescently labeled chorioallantoic membrane (CAM) in the developing G. gallus embryo. HiLo endomicroscopy is a candidate technique for low-cost, high-speed clinical optical biopsies.

Citing Articles

Optical sectioning methods in three-dimensional bioimaging.

Zhang J, Qiao W, Jin R, Li H, Gong H, Chen S Light Sci Appl. 2024; 14(1):11.

PMID: 39741128 PMC: 11688461. DOI: 10.1038/s41377-024-01677-x.

HiLo microscopy with caustic illumination.

Hu G, Greene J, Zhu J, Yang Q, Zheng S, Li Y Biomed Opt Express. 2024; 15(7):4101-4110.

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In Vivo Intelligent Fluorescence Endo-Microscopy by Varifocal Meta-Device and Deep Learning.

Chia Y, Liao W, Vyas S, Chu C, Yamaguchi T, Liu X Adv Sci (Weinh). 2024; 11(20):e2307837.

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