Double-pulse Laser Illumination Method for Measuring Fast Cerebral Blood Flow Velocities in the Deep Brain Using a Fiber-bundle-based Endomicroscopy System

Overview

Journal Biomed Opt Express

Specialty Radiology

Date 2018 Sep 28

PMID 30258684

Citations 2

Authors

Minkyung Kim

Jinki Hong

Hyun-Joon Shin

Affiliations

Soon will be listed here.

Abstract

We present a new fiber-bundle-based endomicroscopy system to measure the fast cerebral blood flow (CBF) velocity in blood vessels located between the surface and the deep brain of living animals. The CBF velocity is obtained by measuring the displacement of the partially overlapped red blood cell images directly, using double-pulse 532-nm laser illumination. The proposed method could measure CBF in blood vessels with diameters ranging from 4 μm to 42 μm and could measure CBF velocities up to 3.2 μm/ms for different vessel diameters at a depth of 2.1 mm from the brain surface in a living mouse.

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References

Secchi M, Sulli A, Pizzorni C, Cutolo M . [Nailfold capillaroscopy and blood flow laser-doppler analysis of the microvascular damage in systemic sclerosis: preliminary results]. Reumatismo. 2009; 61(1):34-40. DOI: 10.4081/reumatismo.2009.34. View

Rosengarten B, Dannhardt V, Burr O, Pohler M, Rosengarten S, Oechsner M . Neurovascular coupling in Parkinson's disease patients: effects of dementia and acetylcholinesterase inhibitor treatment. J Alzheimers Dis. 2010; 22(2):415-21. DOI: 10.3233/JAD-2010-101140. View

Nakamura S, Walker D, Wong F . Cerebral haemodynamic response to somatosensory stimulation in near-term fetal sheep. J Physiol. 2016; 595(4):1289-1303. PMC: 5309357. DOI: 10.1113/JP273163. View

Ford T, Lim D, Mertz J . Fast optically sectioned fluorescence HiLo endomicroscopy. J Biomed Opt. 2012; 17(2):021105. PMC: 3382350. DOI: 10.1117/1.JBO.17.2.021105. View

Cheng H, Luo Q, Liu Q, Lu Q, Gong H, Zeng S . Laser speckle imaging of blood flow in microcirculation. Phys Med Biol. 2004; 49(7):1347-57. DOI: 10.1088/0031-9155/49/7/020. View

Iadecola C . Neurovascular regulation in the normal brain and in Alzheimer's disease. Nat Rev Neurosci. 2004; 5(5):347-60. DOI: 10.1038/nrn1387. View

Mohiaddin R, Firmin D, LONGMORE D . Age-related changes of human aortic flow wave velocity measured noninvasively by magnetic resonance imaging. J Appl Physiol (1985). 1993; 74(1):492-7. DOI: 10.1152/jappl.1993.74.1.492. View

Sugii Y, Nishio S, Okamoto K . In vivo PIV measurement of red blood cell velocity field in microvessels considering mesentery motion. Physiol Meas. 2002; 23(2):403-16. DOI: 10.1088/0967-3334/23/2/315. View

Slaaf D, Jeurens T, Tangelder G, Reneman R, Arts T . Methods to measure blood flow velocity of red blood cells in vivo at the microscopic level. Ann Biomed Eng. 1986; 14(2):175-86. DOI: 10.1007/BF02584268. View

10.

Tomita M, Osada T, Schiszler I, Tomita Y, Unekawa M, Toriumi H . Automated method for tracking vast numbers of FITC-labeled RBCs in microvessels of rat brain in vivo using a high-speed confocal microscope system. Microcirculation. 2008; 15(2):163-74. DOI: 10.1080/10739680701567089. View

11.

Kazmi S, Parthasarthy A, Song N, Jones T, Dunn A . Chronic imaging of cortical blood flow using Multi-Exposure Speckle Imaging. J Cereb Blood Flow Metab. 2013; 33(6):798-808. PMC: 3677120. DOI: 10.1038/jcbfm.2013.57. View

12.

Berezowski V, Fukuda A, Cecchelli R, Badaut J . Endothelial cells and astrocytes: a concerto en duo in ischemic pathophysiology. Int J Cell Biol. 2012; 2012:176287. PMC: 3388591. DOI: 10.1155/2012/176287. View

13.

Letourneur A, Chen V, Waterman G, Drew P . A method for longitudinal, transcranial imaging of blood flow and remodeling of the cerebral vasculature in postnatal mice. Physiol Rep. 2014; 2(12). PMC: 4332216. DOI: 10.14814/phy2.12238. View

14.

Manning T, Monteiro-Riviere N, Bristol D, Riviere J . Cutaneous laser-Doppler velocimetry in nine animal species. Am J Vet Res. 1991; 52(12):1960-4. View

15.

Turner D, Adamson D . Neuronal-astrocyte metabolic interactions: understanding the transition into abnormal astrocytoma metabolism. J Neuropathol Exp Neurol. 2011; 70(3):167-76. PMC: 3045672. DOI: 10.1097/NEN.0b013e31820e1152. View

16.

Magistretti P, Allaman I . A cellular perspective on brain energy metabolism and functional imaging. Neuron. 2015; 86(4):883-901. DOI: 10.1016/j.neuron.2015.03.035. View

17.

Yizhar O, Fenno L, Davidson T, Mogri M, Deisseroth K . Optogenetics in neural systems. Neuron. 2011; 71(1):9-34. DOI: 10.1016/j.neuron.2011.06.004. View

18.

Rungta R, Osmanski B, Boido D, Tanter M, Charpak S . Light controls cerebral blood flow in naive animals. Nat Commun. 2017; 8:14191. PMC: 5290324. DOI: 10.1038/ncomms14191. View

19.

Vogel A, Lauterborn W . Time-resolved particle image velocimetry used in the investigation of cavitation bubble dynamics. Appl Opt. 2010; 27(9):1869-76. DOI: 10.1364/AO.27.001869. View

20.

Kimbrough I, Robel S, Roberson E, Sontheimer H . Vascular amyloidosis impairs the gliovascular unit in a mouse model of Alzheimer's disease. Brain. 2015; 138(Pt 12):3716-33. PMC: 5006220. DOI: 10.1093/brain/awv327. View