Single-scan HiLo with Line-illumination Strategy for Optical Section Imaging of Thick Tissues

Overview

Journal Biomed Opt Express

Specialty Radiology

Date 2021 May 17

PMID 33996235

Citations 6

Authors

Wei Qiao

Rui Jin

Tianpeng Luo

Yafeng Li

Guoqing Fan

Qingming Luo

Jing Yuan

Affiliations

Soon will be listed here.

Abstract

Optical sectioning has been widely employed for inhibiting out-of-focus backgrounds in three-dimensional (3D) imaging of biological samples. However, point scanning imaging or multiple acquisitions for wide-field optical sectioning in epi-illumination microscopy remains time-consuming for large-scale imaging. In this paper, we propose a single-scan optical sectioning method based on the hybrid illumination (HiLo) algorithm with a line-illumination strategy. Our method combines HiLo background inhibition with confocal slit detection. It thereby offers a higher optical sectioning capability than wide-field HiLo and line-confocal imaging without extra modulation and multiple data acquisition. To demonstrate the optical-sectioning capability of our system, we imaged a thin fluorescent plane and different fluorescence-labeled mouse tissue. Our method shows an excellent background inhibition in thick tissue and thus potentially provides an alternative tool for 3D imaging of large-scale biological tissue.

Citing Articles

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