Shotgun Proteomic Monitoring of Clostridium Acetobutylicum During Stationary Phase of Butanol Fermentation Using Xylose and Comparison with the Exponential Phase

Overview

Journal J Ind Microbiol Biotechnol

Publisher Oxford University Press

Specialty Biotechnology

Date 2012 Mar 8

PMID 22395897

Citations 1

Authors

Kumaran Sivagnanam

Vijaya G S Raghavan

Manesh Shah

Robert L Hettich

Nathan C VerBerkmoes

Mark G Lefsrud

Affiliations

Soon will be listed here.

Abstract

Economically viable production of solvents through acetone-butanol-ethanol (ABE) fermentation requires a detailed understanding of Clostridium acetobutylicum. This study focuses on the proteomic profiling of C. acetobutylicum ATCC 824 from the stationary phase of ABE fermentation using xylose and compares with the exponential growth by shotgun proteomics approach. Comparative proteomic analysis revealed 22.9% of the C. acetobutylicum genome and 18.6% was found to be common in both exponential and stationary phases. The proteomic profile of C. acetobutylicum changed during the ABE fermentation such that 17 proteins were significantly differentially expressed between the two phases. Specifically, the expression of five proteins namely, CAC2873, CAP0164, CAP0165, CAC3298, and CAC1742 involved in the solvent production pathway were found to be significantly lower in the stationary phase compared to the exponential growth. Similarly, the expression of fucose isomerase (CAC2610), xylulose kinase (CAC2612), and a putative uncharacterized protein (CAC2611) involved in the xylose utilization pathway were also significantly lower in the stationary phase. These findings provide an insight into the metabolic behavior of C. acetobutylicum between different phases of ABE fermentation using xylose.

Citing Articles

A quantitative metabolomics study of high sodium response in Clostridium acetobutylicum ATCC 824 acetone-butanol-ethanol (ABE) fermentation.

Zhao X, Condruz S, Chen J, Jolicoeur M Sci Rep. 2016; 6:28307.

PMID: 27321153 PMC: 4913259. DOI: 10.1038/srep28307.

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