Cytometry: Today's Technology and Tomorrow's Horizons

Overview

Journal Methods

Specialty Biochemistry

Date 2012 Mar 7

PMID 22391486

Citations 61

Authors

Pratip K Chattopadhyay

Mario Roederer

Affiliations

Soon will be listed here.

Abstract

Flow cytometry has been the premier tool for single cell analysis since its invention in the 1960s. It has maintained this position through steady advances in technology and applications, becoming the main force behind interrogating the complexities of the immune system. Technology development was a three-pronged effort, including the hardware, reagents, and analysis algorithms to allow measurement of as many as 20 independent parameters on each cell, at tens of thousands of cells per second. In the coming years, cytometry technology will integrate with other techniques, such as transcriptomics, metabolomics, and so forth. Ongoing efforts are aimed at algorithms to analyse these aggregated datasaets over large numbers of samples. Here we review the development efforts heralding the next stage of flow cytometry.

Citing Articles

Clustering Algorithm-Driven Detection of TRBC1-Restricted Clonal T-Cell Populations Produces Better Results than Manual Gating Analysis.

Bucek S, Brozic A, Miceska S, Gasljevic G, Kloboves Prevodnik V Int J Mol Sci. 2025; 26(1.

PMID: 39796028 PMC: 11720138. DOI: 10.3390/ijms26010170.

Towards personalized vaccines.

Montin D, Santilli V, Beni A, Costagliola G, Martire B, Mastrototaro M Front Immunol. 2024; 15:1436108.

PMID: 39421749 PMC: 11484009. DOI: 10.3389/fimmu.2024.1436108.

A Comprehensive Exploration of Caspase Detection Methods: From Classical Approaches to Cutting-Edge Innovations.

Zhra M, Qasem R, Aldossari F, Saleem R, Aljada A Int J Mol Sci. 2024; 25(10).

PMID: 38791499 PMC: 11121653. DOI: 10.3390/ijms25105460.

Co-staining with Fluorescent Antibodies and Antibody-Derived Tags for Cell Sorting Prior to CITE-Seq.

Shi X, Baracho G, Lomas 3rd W, Song H, Widmann S, Tyznik A Methods Mol Biol. 2024; 2779:287-303.

PMID: 38526791 DOI: 10.1007/978-1-0716-3738-8_13.

SERS characterization of colorectal cancer cell surface markers upon anti-EGFR treatment.

Lyu N, Pedersen B, Shklovskaya E, Rizos H, Molloy M, Wang Y Exploration (Beijing). 2023; 2(3):20210176.

PMID: 37323700 PMC: 10190927. DOI: 10.1002/EXP.20210176.

References

Gaucher D, Therrien R, Kettaf N, Angermann B, Boucher G, Filali-Mouhim A . Yellow fever vaccine induces integrated multilineage and polyfunctional immune responses. J Exp Med. 2008; 205(13):3119-31. PMC: 2605227. DOI: 10.1084/jem.20082292. View

Itoh M, Takahashi T, Sakaguchi N, KUNIYASU Y, Shimizu J, Otsuka F . Thymus and autoimmunity: production of CD25+CD4+ naturally anergic and suppressive T cells as a key function of the thymus in maintaining immunologic self-tolerance. J Immunol. 1999; 162(9):5317-26. View

Chattopadhyay P, Price D, Harper T, Betts M, Yu J, Gostick E . Quantum dot semiconductor nanocrystals for immunophenotyping by polychromatic flow cytometry. Nat Med. 2006; 12(8):972-7. DOI: 10.1038/nm1371. View

Fontenot J, Gavin M, Rudensky A . Foxp3 programs the development and function of CD4+CD25+ regulatory T cells. Nat Immunol. 2003; 4(4):330-6. DOI: 10.1038/ni904. View

Aghaeepour N, Nikolic R, Hoos H, Brinkman R . Rapid cell population identification in flow cytometry data. Cytometry A. 2010; 79(1):6-13. PMC: 3137288. DOI: 10.1002/cyto.a.21007. View

Qiu P, Simonds E, Bendall S, Gibbs Jr K, Bruggner R, Linderman M . Extracting a cellular hierarchy from high-dimensional cytometry data with SPADE. Nat Biotechnol. 2011; 29(10):886-91. PMC: 3196363. DOI: 10.1038/nbt.1991. View

Sun Y, Santra S, Buzby A, Mascola J, Nabel G, Letvin N . Recombinant vector-induced HIV/SIV-specific CD4+ T lymphocyte responses in rhesus monkeys. Virology. 2010; 406(1):48-55. PMC: 2952962. DOI: 10.1016/j.virol.2010.07.004. View

Bendall S, Simonds E, Qiu P, Amir E, Krutzik P, Finck R . Single-cell mass cytometry of differential immune and drug responses across a human hematopoietic continuum. Science. 2011; 332(6030):687-96. PMC: 3273988. DOI: 10.1126/science.1198704. View

Darrah P, Patel D, De Luca P, Lindsay R, Davey D, Flynn B . Multifunctional TH1 cells define a correlate of vaccine-mediated protection against Leishmania major. Nat Med. 2007; 13(7):843-50. DOI: 10.1038/nm1592. View

10.

Chattopadhyay P, Perfetto S, Roederer M . The colorful future of cell analysis by flow cytometry. Discov Med. 2010; 4(23):255-62. View

11.

Precopio M, Betts M, Parrino J, Price D, Gostick E, Ambrozak D . Immunization with vaccinia virus induces polyfunctional and phenotypically distinctive CD8(+) T cell responses. J Exp Med. 2007; 204(6):1405-16. PMC: 2118607. DOI: 10.1084/jem.20062363. View

12.

Roederer M, Nozzi J, Nason M . SPICE: exploration and analysis of post-cytometric complex multivariate datasets. Cytometry A. 2011; 79(2):167-74. PMC: 3072288. DOI: 10.1002/cyto.a.21015. View

13.

Roederer M, Moore W, Treister A, Hardy R, Herzenberg L . Probability binning comparison: a metric for quantitating multivariate distribution differences. Cytometry. 2001; 45(1):47-55. DOI: 10.1002/1097-0320(20010901)45:1<47::aid-cyto1143>3.0.co;2-a. View

14.

De Rosa S, Herzenberg L, Roederer M . 11-color, 13-parameter flow cytometry: identification of human naive T cells by phenotype, function, and T-cell receptor diversity. Nat Med. 2001; 7(2):245-8. DOI: 10.1038/84701. View

15.

Chattopadhyay P, Hogerkorp C, Roederer M . A chromatic explosion: the development and future of multiparameter flow cytometry. Immunology. 2009; 125(4):441-9. PMC: 2612557. DOI: 10.1111/j.1365-2567.2008.02989.x. View

16.

Roederer M . Spectral compensation for flow cytometry: visualization artifacts, limitations, and caveats. Cytometry. 2001; 45(3):194-205. DOI: 10.1002/1097-0320(20011101)45:3<194::aid-cyto1163>3.0.co;2-c. View

17.

Thornton A, Shevach E . CD4+CD25+ immunoregulatory T cells suppress polyclonal T cell activation in vitro by inhibiting interleukin 2 production. J Exp Med. 1998; 188(2):287-96. PMC: 2212461. DOI: 10.1084/jem.188.2.287. View

18.

Hulett H, Bonner W, Barrett J, Herzenberg L . Cell sorting: automated separation of mammalian cells as a function of intracellular fluorescence. Science. 1969; 166(3906):747-9. DOI: 10.1126/science.166.3906.747. View

19.

Crotty S . Follicular helper CD4 T cells (TFH). Annu Rev Immunol. 2011; 29:621-63. DOI: 10.1146/annurev-immunol-031210-101400. View

20.

Chattopadhyay P . Quantum dot technology in flow cytometry. Methods Cell Biol. 2011; 102:463-77. DOI: 10.1016/B978-0-12-374912-3.00018-3. View